Evidence against a major role for TKTL-1 in hypoxic and normoxic cancer cells.

Abstract

Based on studies performed with a selected mouse monoclonal anti-transketolase- like (TKTL)-1 antibody (clone JFC12T10), overexpression of TKTL-1 has been shown to be correlated with poor survival and increased metastatic spread in several human tumor entities. Since the clinical aggressiveness mediated by TKTL-1 has been partially related to resistance to hypoxia,we originally aimed to explore the influence of hypoxia on the expression of TKTL-1. Unexpectedly, results of our experiments indicated that the antibody clone JFC12T10 lacks target specificity. Since the majority of data on the role of TKTL-1 in human cancer is based upon studies performed with this antibody clone, we subsequently re-evaluated the expression of TKTL-1 in six different cancer cell lines (HeLa, MCF-7, A549, HT-1080, M21 and TF-1). Using RT-PCR and consecutive sequence analysis, we show that transketolase (TKT), not TKTL-1, is the dominant isoform of transketolases in the cell lines analyzed. Our data argue against a major role of TKTL-1 for the metabolism of cancer cells.

• Authors: Arnulf Mayer, Angelika von Wallbrunn, Peter Vaupel
• Journal: Advances in experimental medicine and biology (Adv Exp Med Biol) Vol. 701 Pg. 123-8 ( 2011) ISSN: 0065-2598 [Print] United States
• PMID: 21445778 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• RNA, Messenger
• Transketolase
• Oxygen

Topics

• Animals
• Antibodies, Monoclonal (immunology, metabolism)
• Blotting, Western
• Cell Nucleus (enzymology)
• Cytoplasm (enzymology)
• Humans
• Hypoxia
• Immunoenzyme Techniques
• Mice
• Neoplasms (enzymology, pathology)
• Oxygen (metabolism)
• RNA, Messenger (genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Transketolase (genetics, immunology, metabolism)
• Tumor Cells, Cultured