Alteration of interferon-α/β receptors in chronic hepatitis B patients.

Abstract

BACKGROUND:

The present study determined type I interferon (IFN) receptor (interferon-α/β receptor (IFNAR)) and its predicable role in interferon-α2b treatment in chronic hepatitis B (CHB) patients.

METHODS:

Expression of IFN-α/βR-1 and IFN-α/βR-2 in peripheral blood mononuclear cells and in liver tissue was measured by flow cytometry, immunofluorescence, immunohistochemistry and RT-PCR.

RESULTS:

IFN-α/βR-1 and IFN-α/βR-2 in monocytes and lymphocytes increased in CHB patients. Expression of IFNAR-1 and IFNAR-2 in liver had positive correlation with HBV-DNA in liver tissue. Expression of IFN-α/βR in lymphocytes and monocytes increased in the first month, but then decreased during the subsequent interferon-α2b treatment, patients who had higher levels of IFN-α/βR-2 in monocytes prior to therapy showed better viral response than those with lower levels.

CONCLUSIONS:

Expression of IFN-α/βR-2 in monocytes can be used as a predictable parameter to evaluate the effect of IFN-α treatment in CHB patients.

• Authors: Fanli Meng, Jiefei Wang, Jian Ge, Xiaopeng Fan, Bing Wang, Liyan Han, Tatiana Kisseleva, YongHan Paik, David A Brenner, Kai Wang
• Journal: Journal of clinical immunology (J Clin Immunol) Vol. 31 Issue 3 Pg. 521-32 (Jun 2011) ISSN: 1573-2592 [Electronic] Netherlands
• PMID: 21445562 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA, Viral
• Hepatitis B Surface Antigens
• Hepatitis B e Antigens
• Interferon-alpha
• Receptor, Interferon alpha-beta

Topics

• Adult
• Case-Control Studies
• DNA, Viral (blood, immunology)
• Female
• Flow Cytometry
• Fluorescent Antibody Technique
• Hepatitis B Surface Antigens (blood)
• Hepatitis B e Antigens (blood)
• Hepatitis B virus (drug effects, immunology, physiology)
• Hepatitis B, Chronic (blood, drug therapy, immunology, pathology, virology)
• Humans
• Immunohistochemistry
• Interferon-alpha (administration & dosage, therapeutic use)
• Leukocytes, Mononuclear (cytology, drug effects, immunology)
• Male
• Middle Aged
• Receptor, Interferon alpha-beta (blood, genetics, immunology)
• Reverse Transcriptase Polymerase Chain Reaction
• Viral Load (drug effects)