Concentration and purification of rubella virus using monolithic chromatographic support.

Abstract

The production of economically acceptable viral vaccines of high quality requires simple and efficient methods for purification and concentration of viral particles. Ion-exchange chromatography (IEC) has become one of commonly used methods for large-scale downstream purification of viruses. Viruses possess different biological and/or biochemical properties and therefore IEC conditions must be established specifically for each virus. Live attenuated rubella virus vaccines have been manufactured and successfully used widely to protect people from rubella and congenital rubella syndrome for almost 40 years. The aim of this study was to search for an efficient method for concentration and purification of rubella virus using IEC. The selected operating conditions using quaternary amine monolithic supports enabled highly efficient binding, purification and concentration of rubella virus from complex biological suspension without additional procedures. Eluted viral particles maintained their infectivity and viral recovery was almost 100%. At the same time, viral preparation was successfully depleted from host cell protein and DNA. This work indicates the possibility of using monoliths to improve the rubella virus yields in productions where high virus titers during cultivation can hardly be achieved.

• Authors: Dubravko Forcic, Marija Brgles, Jelena Ivancic-Jelecki, Maja Santak, Beata Halassy, Miloš Barut, Renata Jug, Maja Markušić, Aleš Strancar
• Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 879 Issue 13-14 Pg. 981-6 (Apr 15 2011) ISSN: 1873-376X [Electronic] Netherlands
• PMID: 21440513 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Rubella Vaccine

Topics

• Cell Line
• Chromatography, Ion Exchange (methods)
• Humans
• Reproducibility of Results
• Rubella Vaccine (chemical synthesis, immunology)
• Rubella virus (immunology, isolation & purification)
• Virion (immunology, isolation & purification)
• Virus Cultivation