CXC chemokine receptor 1 enhances the ability of human umbilical cord blood-derived mesenchymal stem cells to migrate toward gliomas.

Abstract

In this study, we showed that knocking-down interleukin-8 (IL-8) in glioma cells, or its receptor, CXC chemokine receptor 1 (CXCR1) in hUCB-MSCs reduced hUCB-MSC migration toward glioma cells in a Transwell chamber. In contrast, CXCR1-transfected hUCB-MSCs (CXCR1-MSCs) showed a superior capacity to migrate toward glioma cells in a Transwell chamber compared to primary hUCB-MSCs. Furthermore, these transfected cells also demonstrated the same ability to migrate toward tumors in mice bearing intracranial human gliomas as shown by histological and in vivo imaging analysis. Our findings indicate that overexpression of CXCR1 could be a useful tool for MSC-based gene therapy to achieve a sufficient quantity of therapeutic MSCs that are localized within tumors.

• Authors: Seong Muk Kim, Dal-Soo Kim, Chang Hyun Jeong, Dong Hyun Kim, Ji Hyun Kim, Hong Bae Jeon, Soon-Jae Kwon, Sin-Soo Jeun, Yoon Sun Yang, Wonil Oh, Jong Wook Chang
• Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 407 Issue 4 Pg. 741-6 (Apr 22 2011) ISSN: 1090-2104 [Electronic] United States
• PMID: 21439934 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Inc. All rights reserved.

Chemical References

• Interleukin-8
• Receptors, Interleukin-8A

Topics

• Animals
• Brain Neoplasms (therapy)
• Cell Line, Tumor
• Cell Movement
• Fetal Blood (cytology)
• Gene Knockdown Techniques
• Genetic Therapy (methods)
• Glioma (therapy)
• Humans
• Interleukin-8 (genetics)
• Mesenchymal Stem Cell Transplantation
• Mesenchymal Stem Cells (metabolism, physiology)
• Mice
• Mice, Nude
• Receptors, Interleukin-8A (genetics)
• Transfection
• Xenograft Model Antitumor Assays