Abstract |
Despite intensive efforts to improve therapies, small cell lung cancer (SCLC) still has a dismal median survival of 18 months. Since miR-126 is under-expressed in the majority of SCLC tumors, we investigated the effect of miR-126 overexpression on the proliferation and cell cycle distribution of H69 cells. Our results demonstrate that miR-126 inhibits proliferation of H69 cells, by delaying the cells in the G1 phase. Short interfering RNA ( siRNA) mediated suppression of SLC7A5, a predicted target of mir-126, has the same effect on H69 cells. We also show for the first time that SLC7A5 is a direct target of miR-126.
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Authors | Edit Miko, Zoltán Margitai, Zsolt Czimmerer, Ildikó Várkonyi, Balázs Dezso, Arpád Lányi, Zsolt Bacsó, Beáta Scholtz |
Journal | FEBS letters
(FEBS Lett)
Vol. 585
Issue 8
Pg. 1191-6
(Apr 20 2011)
ISSN: 1873-3468 [Electronic] England |
PMID | 21439283
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. |
Chemical References |
- Large Neutral Amino Acid-Transporter 1
- MIRN126 microRNA, human
- MicroRNAs
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Topics |
- Blotting, Western
- Cell Line, Tumor
- Cell Proliferation
- G1 Phase
(genetics)
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- Large Neutral Amino Acid-Transporter 1
(genetics, metabolism)
- Lung Neoplasms
(genetics, metabolism, pathology)
- MicroRNAs
(genetics)
- RNA Interference
- Reverse Transcriptase Polymerase Chain Reaction
- Small Cell Lung Carcinoma
(genetics, metabolism, pathology)
- Time Factors
- Tissue Array Analysis
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