miR-126 inhibits proliferation of small cell lung cancer cells by targeting SLC7A5.

Abstract

Despite intensive efforts to improve therapies, small cell lung cancer (SCLC) still has a dismal median survival of 18 months. Since miR-126 is under-expressed in the majority of SCLC tumors, we investigated the effect of miR-126 overexpression on the proliferation and cell cycle distribution of H69 cells. Our results demonstrate that miR-126 inhibits proliferation of H69 cells, by delaying the cells in the G1 phase. Short interfering RNA (siRNA) mediated suppression of SLC7A5, a predicted target of mir-126, has the same effect on H69 cells. We also show for the first time that SLC7A5 is a direct target of miR-126.

• Authors: Edit Miko, Zoltán Margitai, Zsolt Czimmerer, Ildikó Várkonyi, Balázs Dezso, Arpád Lányi, Zsolt Bacsó, Beáta Scholtz
• Journal: FEBS letters (FEBS Lett) Vol. 585 Issue 8 Pg. 1191-6 (Apr 20 2011) ISSN: 1873-3468 [Electronic] England
• PMID: 21439283 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

Chemical References

• Large Neutral Amino Acid-Transporter 1
• MIRN126 microRNA, human
• MicroRNAs

Topics

• Blotting, Western
• Cell Line, Tumor
• Cell Proliferation
• G1 Phase (genetics)
• Gene Expression Regulation, Neoplastic
• Humans
• Immunohistochemistry
• Large Neutral Amino Acid-Transporter 1 (genetics, metabolism)
• Lung Neoplasms (genetics, metabolism, pathology)
• MicroRNAs (genetics)
• RNA Interference
• Reverse Transcriptase Polymerase Chain Reaction
• Small Cell Lung Carcinoma (genetics, metabolism, pathology)
• Time Factors
• Tissue Array Analysis