POTE
ankyrin domain family, member H (POTEH) belongs to POTE family, which expresses in many
cancers. In this study, methylation status of POTEH promoter and its correlation with clinicopathological parameters were evaluated in
glioma tissues and cells.
Bisulfite sequencing PCR was carried out to investigate the promoter methylation status of POTEH in
tumor of 96
glioma patients and
glioma cells U251, SF767, and SF126. The effect of promoter hypomethylation on
protein expression was evaluated by immunohistochemistry. POTEH was hypomethylated in 81.3%
gliomas and none in normal brain tissues, and correlated significantly with its
protein expression. But there was no remarkable relationship between sex, age, advanced
tumor grade and POTEH hypomethylation. With the grade progressing, POTEH
protein expression was enhanced. The correlation between POTEH hypomethylation,
protein expression and overall survival was statistically significant. In POTEH hypomethylation group, patients with POTEH high expression had shorter overall survival than those with low expression. Hypomethylation of POTEH promoter in
gliomas accounted for POTEH
protein overexpression and poor outcome in a subset of patients. Detection of these epigenetic changes in
tumors may provide information regarding prognosis.