Abstract | OBJECTIVE: To study the total glucosides of Radix Paeoniae Rubra induced K562 tumor cell apoptosis of signaling pathways and related gene changes. METHOD: By MTT and flow cytometric, real-time quantitatie polymerase chain reaction (PCR) and Western blot methods researched the level of genes and proteins. RESULT: The total glucosides of Radix Paeoniae Rubra could inhibit K562 cell growth by MTT method, there was the relationship of concentration-time between them, TGC prompted K562 cell translocation of phosphatidylserine, may be apoptosis through non-receptor-dependent pathways because caspase-3 mRNA, caspase-9 mRNA and cytochrome C increased, caspase-8 mRNA had no significant change. The expression of Bcl-2 protein and Bcl-X1 protein could decreased, the expression of Bax protein could increased by regulating gene expression. CONCLUSION: TGC induced K562 cell apoptosis that might be through the mitochondria pathway, when cell apoptosis occured, Bcl-2 or Bcl-XI proteins combination of Bax protein would be displacement, then the mitochondrial membrane became permeable to release a series of material, then lead to cell death.
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Authors | Huiyu Xu, Zhiwei Chen, Li Zhou, Jianzhao Niu |
Journal | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica
(Zhongguo Zhong Yao Za Zhi)
Vol. 35
Issue 24
Pg. 3377-81
(Dec 2010)
ISSN: 1001-5302 [Print] China |
PMID | 21438411
(Publication Type: English Abstract, Journal Article)
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Chemical References |
- Antineoplastic Agents
- Glucosides
- Caspases
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Topics |
- Antineoplastic Agents
(pharmacology)
- Apoptosis
(drug effects, genetics)
- Caspases
(genetics)
- Dose-Response Relationship, Drug
- Gene Expression Regulation, Neoplastic
(drug effects)
- Glucosides
(pharmacology)
- Humans
- K562 Cells
- Paeonia
(chemistry)
- Signal Transduction
(drug effects)
- Time Factors
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