Abstract | OBJECTIVE: METHODS: 18 male SD rats were randomly divided into 3 groups, 6 in each group. After the models were established, the rats were treated with SA-B for 2 weeks. Then their renal pathology were examined by hight microscope and electron microscopy Protein expression levels of alpha-smooth muscle actin (alpha-SMA) and E-cadherin (E-cad) in the obstructed kidney were analyzed by Western blot and Biochemistry assay. RESULTS: Pathological examination of the kidney in model group showed tubules lumen widened and many inflammatory cells infiltrated, a part of renal tubule expanded and part of them atrophied. The tubular epithelial cells were karyorrhexis or karyolysis, some tubulars were atrophy. The protein expression of alpha-SMA were significantly up-regulated (P < 0.01) and E-cad were significantly down-regulated (P < 0.01) in the model group. After intervention with SA-B, the renal pathological status in the treatment group was significantly improved, the expression of alpha-SMA were significantly down-regulated (P < 0.05), but E-cad only a little up-regulated (P > 0.05). CONCLUSION: SA-B could antagonize renal interstitial fibrosis mainly by maintaining epithelial phenotype, inhibiting the protein of alpha-SMA which is the principal effect cells that are responsible for the progressive kidney fibrosis.
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Authors | Hai-Ying Lu, Juan Zhou, Min Lu, Yu-Min Liu, Fei Wang, Min Lin, Yue Zhang |
Journal | Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
(Zhong Yao Cai)
Vol. 33
Issue 11
Pg. 1755-9
(Nov 2010)
ISSN: 1001-4454 [Print] China |
PMID | 21434439
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Actins
- Benzofurans
- Cadherins
- salvianolic acid B
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Topics |
- Actins
(metabolism)
- Animals
- Benzofurans
(pharmacology, therapeutic use)
- Blotting, Western
- Cadherins
(metabolism)
- Disease Models, Animal
- Fibrosis
(drug therapy, pathology)
- Immunohistochemistry
- Kidney
(metabolism, pathology)
- Kidney Diseases
(drug therapy, etiology, pathology)
- Kidney Tubules
(metabolism, pathology)
- Male
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Ureteral Obstruction
(complications)
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