[Anti-sense miRNA-21 oligonucleotide inhibits Tb 3.1 human tongue squamous cell carcinoma growth in vitro].

Abstract

OBJECTIVE:

To investigate the effect of micro RNA-21 (miRNA-21) knocking on the Tb3.1 human tongue squamous cell carcinoma growth.

METHODS:

Anti-sense miRNA-21 oligonucleotide was delivered with oligofectamine to suppress Tb 3.1 tongue cancer cell growth in vitro. Real-time polymerase chain reaction (PCR) was conducted to detect the miRNA-21 expression after transfection. Methyl thiazolyl tetrazolium (MTT) assay was used to determine Tb 3.1 cell survival rate. Apoptosis were examined by flow-cytometry. Matrigel matrix and transwell assay were used to determine Tb 3.1 cell colony formation and migration ability. Antigen KI-67 (Ki67), B cell lymphoma (Bcl-2), phosphatase and tensin homolog (PTEN), matrirx metalloproteinase 2 (MMP-2, MMP-9) and tissue inhibitor of metalloproteinase 1 (TIMP-1) protein expression in Tb 3.1 cell were measured by Western blotting.

RESULTS:

miRNA-21 expression was decreased in miRNA-21 antisense oligonucleotide (ASODN) group. The survival rate of Tb 3.1 cells with AS-miRNA-21 transfection was significantly suppressed (F = 27.02, P = 0.00) and early phase apoptosis (F = 26.641, P = 0.001) induced in Tb 3.1 cell. Ki67, Bcl-2, MMP-2 and MMP-9 protein were down regulated while PTEN and TIMP-1 protein expression was increased.

CONCLUSIONS:

Blocking miRNA-21 expression in Tb3.1 cell could suppress cancer cell growth in vitro and miRNA-21 can serve as a novel target candidate for human tongue cancer gene therapy.

• Authors: Ying-jie Tao, Yu Ren, Jia-bin Dong, Lun Zhang, Jun-ping Cheng, Xuan Zhou
• Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (Zhonghua Kou Qiang Yi Xue Za Zhi) Vol. 46 Issue 2 Pg. 79-83 (Feb 2011) ISSN: 1002-0098 [Print] China
• PMID: 21426775 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Ki-67 Antigen
• MIRN21 microRNA, human
• MicroRNAs
• Oligonucleotides, Antisense
• Proto-Oncogene Proteins c-bcl-2
• TIMP1 protein, human
• Tissue Inhibitor of Metalloproteinase-1
• PTEN Phosphohydrolase
• PTEN protein, human
• MMP2 protein, human
• Matrix Metalloproteinase 2
• MMP9 protein, human
• Matrix Metalloproteinase 9

Topics

• Apoptosis
• Carcinoma, Squamous Cell (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• Down-Regulation
• Humans
• Ki-67 Antigen (metabolism)
• Matrix Metalloproteinase 2 (metabolism)
• Matrix Metalloproteinase 9 (metabolism)
• MicroRNAs (genetics, metabolism)
• Oligonucleotides, Antisense (genetics)
• PTEN Phosphohydrolase (metabolism)
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Tissue Inhibitor of Metalloproteinase-1 (metabolism)
• Tongue Neoplasms (genetics, metabolism, pathology)
• Transfection