The effect of cytostatics and hyperthermia on RAJI human lymphoma cells.

Abstract

AIM:

To evaluate the effect of hyperthermia on cytostatic activity of chemotherapeutic drugs carboplatin, cisplatin, oxaliplatin, carmustine, gemcitabine and etoposide in human lymphoma cell culture.

METHODS:

RAJI human lymphoma cells were incubated with cytostatics at 37 °C or 42 °C and evaluated for cell culture growth.

RESULTS:

The number of viable cells after incubation with the drugs (except for gemcitabine) at 42 °C for 30 min was significantly lower than at 37 °C. There were synergism of cytostatic effects of platinum drugs (carboplatin, cisplatin and oxaliplatin) with cytostatic effect of 42 °C and the summation of cytostatic effect of carmustine or etoposide with the action of hyperthermia. The thermal enhancement ratio was 3.0 for oxaliplatin, 2.0 for cisplatin, 1.8-2.4 for carboplatin. The combination of platinum drugs with gemcitabine resulted in a significant enhancement of cytostatic activity.

CONCLUSION:

Our findings suggest that a gain in sensitivity of RAJI human lymphoma cells to platinum drugs occurs at 42 °C.

• Authors: Yu P Istomin, E A Zhavrid, N V Sachivko, E N Alexandrova, P V Pocheshinsky
• Journal: Experimental oncology (Exp Oncol) Vol. 33 Issue 1 Pg. 19-23 (Mar 2011) ISSN: 1812-9269 [Print] Ukraine
• PMID: 21423090 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Cytostatic Agents

Topics

• Antineoplastic Agents (pharmacology)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Survival (drug effects)
• Cytostatic Agents (pharmacology)
• Hot Temperature
• Humans