Abstract |
Ethylene glycol monomethyl ether (EGME) is a known reproductive toxicant that induces luteal hypertrophy in rat ovaries. In this study, we characterized the histopathological features of corpora lutea (CL) from EGME-treated rats and compared them with normal CL formation and regression. Normally cycling female Sprague-Dawley rats were treated with 5-bromo-2'-deoxyuridine ( BrdU) intraperitoneally on the morning of estrus and their ovaries were examined 1 (metestrus), 4 (estrus), 8 (estrus), or 12 (estrus) days later to observe the transition of BrdU-labeled cells within in the CL. CL at each time point of estrus stage were classified into 4 types: Type I (newly formed CL), Type II (mature CL), Type III (regressing CL), and Type IV (residual CL). CL almost fully regressed within 4 estrus cycles. In contrast, in female rats given EGME orally (30, 100, or 300 mg/kg for 2 or 4 weeks), luteal cells were hypertrophic with abundant cytoplasm. Although the size of CL varied, all CL in EGME-treated rats had histological features similar to Type II CL, but they were more hypertrophic with less apoptosis. These results suggest that EGME has a luteal hypertrophic effect on all CL phases, including regression.
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Authors | Yoshikazu Taketa, Akira Inomata, Satoru Hosokawa, Jiro Sonoda, Kazuhiro Hayakawa, Kyoko Nakano, Yuki Momozawa, Joji Yamate, Midori Yoshida, Toyohiko Aoki, Kazuo Tsukidate |
Journal | Toxicologic pathology
(Toxicol Pathol)
Vol. 39
Issue 2
Pg. 372-80
(Feb 2011)
ISSN: 1533-1601 [Electronic] United States |
PMID | 21422263
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Ethylene Glycols
- Progesterone
- methyl cellosolve
- Bromodeoxyuridine
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Topics |
- Animals
- Bromodeoxyuridine
(metabolism)
- Estrus
(drug effects)
- Ethylene Glycols
(toxicity)
- Female
- Hypertrophy
(chemically induced, pathology)
- Luteal Cells
(drug effects, pathology)
- Luteolysis
(drug effects)
- Models, Animal
- Ovary
(drug effects, pathology)
- Progesterone
(metabolism)
- Rats
- Rats, Sprague-Dawley
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