Analysis of insertion/deletion polymorphisms of the angiotensin converting enzyme gene in Malaysian end-stage renal disease patients.

Abstract

INTRODUCTION:

Insertion/deletion (I/D) polymorphisms found in the angiotensin converting enzyme (ACE) gene have been associated with hypertension, diabetes and renal disease. The present study sought to determine the association of I/D polymorphisms of the ACE gene with end-stage renal disease (ESRD) patients in Malaysia.

MATERIALS AND METHODS:

A total of 380 subjects were recruited to determine the genotypes of I/D polymorphisms of the ACE gene. Genotyping was performed using a PCR method. Statistical analyses were carried out using statistical software, and a level of p < 0.05 was considered statistically significant.

RESULTS:

The frequencies for II, ID and DD genotypes of the ACE gene were 24.7%, 65.80% and 9.47%, respectively, in ESRD patients, and in control subjects were 45.26%, 47.37% and 7.37% respectively. The frequency for the D allele was found to be higher (42.40%) in ESRD patients compared to control subjects (31.05%). The genotypic and allelic frequencies of I/D polymorphisms of the ACE gene differed significantly (p < 0.05) between ESRD patients and control subjects in the Malaysian population.

CONCLUSION:

The findings of this study indicate that I/D polymorphisms of the ACE gene are a useful marker and are likely to play a major role in determining genetic susceptibility to ESRD in the Malaysian population.

• Authors: Aisyah Ali, Ramachandran Vasudevan, Patimah Ismail, Christoper Lim Thiam Seong, Srikumar Chakravarthi
• Journal: Journal of the renin-angiotensin-aldosterone system : JRAAS (J Renin Angiotensin Aldosterone Syst) Vol. 16 Issue 4 Pg. 1337-43 (Dec 2015) ISSN: 1752-8976 [Electronic] England
• PMID: 21421653 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: © The Author(s) 2011.

Chemical References

• ACE protein, human
• Peptidyl-Dipeptidase A

Topics

• Adult
• Aged
• Case-Control Studies
• Electrophoresis, Agar Gel
• Female
• Gene Frequency (genetics)
• Genetic Predisposition to Disease
• Heterozygote
• Humans
• INDEL Mutation (genetics)
• Kidney Failure, Chronic (enzymology, genetics)
• Malaysia
• Male
• Middle Aged
• Peptidyl-Dipeptidase A (genetics)
• Polymorphism, Genetic