Abstract | BACKGROUND AND AIM:
Oridonin is the active ingredient isolated from the Chinese herb Rabdosia rubescens. We used both in vivo and in vitro approaches to elucidate the underlying mechanism of the oridonin-mediated inhibition of colorectal cancer. METHODS: RESULTS: The treatment of Lovo and SW480 cells with oridonin inhibited cell proliferation in a dose-dependent manner. Furthermore, the rate of inhibition increased with prolonged treatment. The growth rate of the colorectal cancer colostomy implantation model was significantly lower than control cells when treated with oridonin (P<0.001), which meant that oridonin treatment had a significant effect on the tumor growth rate. In the tumor model, activator protein-1 (AP-1) was the only gene found to be downregulated after oridonin treatment by the gene expression sensor. After 4 weeks of treatment, AP-1, nuclear factor-κB (NF-κB) and P38 were all found to be downregulated. CONCLUSIONS:
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Authors | Heiying Jin, Xuanzhong Tan, Xiufang Liu, Yijiang Ding |
Journal | Journal of gastroenterology and hepatology
(J Gastroenterol Hepatol)
Vol. 26
Issue 4
Pg. 706-15
(Apr 2011)
ISSN: 1440-1746 [Electronic] Australia |
PMID | 21418301
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2011 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd. |
Chemical References |
- Antineoplastic Agents, Phytogenic
- Diterpenes, Kaurane
- NF-kappa B
- Transcription Factor AP-1
- oridonin
- p38 Mitogen-Activated Protein Kinases
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Topics |
- Antineoplastic Agents, Phytogenic
(pharmacology)
- Apoptosis
(drug effects)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Colorectal Neoplasms
(drug therapy, genetics, pathology)
- Diterpenes, Kaurane
(pharmacology)
- Dose-Response Relationship, Drug
- Down-Regulation
- Flow Cytometry
- Humans
- NF-kappa B
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
- Time Factors
- Transcription Factor AP-1
(genetics)
- Tumor Burden
(drug effects)
- Xenograft Model Antitumor Assays
- p38 Mitogen-Activated Protein Kinases
(genetics)
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