Insulin induces fatty acid desaturase expression in human monocytes.

Abstract

Increasing evidence suggests that fatty acid desaturases, rate-limiting enzymes in unsaturated fatty acid biosynthesis, are important factors in the pathogenesis of lipid-induced insulin resistance. The conversion of dihomogamma linolenic acid (DGLA) into arachidonic acid (AA) in human plasma phospholipids has been shown to be regulated by insulin, suggesting a role for insulin in fatty acid desaturase 1 regulation. However insulin's role in monocyte inflammation associated with obesity and lifestyle disease development is uncertain. We therefore investigated if insulin is able to induce expression of stearoyl-CoA desaturase (SCD, Δ9 desaturase), fatty acid desaturase 1 (FADS1, Δ5 desaturase), and fatty acid desaturase 2 (FADS2, Δ6 desaturase), as well as the sterol regulatory element binding transcription factor 1-c (SREBP-1c) in monocytes. Here, for the first time, we demonstrate that THP-1 monocytes are insulin-responsive in inducing expression of SCD, FADS1, and FADS2 in a time- and dose-dependent manner. Understanding secondary consequences of postprandial hyperinsulinemia may open up new strategies for prevention and/or treatment of obesity-related metabolic complications.

• Authors: Ingerid Arbo, Cathinka Halle, Darshan Malik, Hans-Richard Brattbakk, Berit Johansen
• Journal: Scandinavian journal of clinical and laboratory investigation (Scand J Clin Lab Invest) Vol. 71 Issue 4 Pg. 330-9 (Jul 2011) ISSN: 1502-7686 [Electronic] England
• PMID: 21413848 (Publication Type: Journal Article)

Chemical References

• Culture Media
• Delta-5 Fatty Acid Desaturase
• Insulin
• Sterol Regulatory Element Binding Protein 1
• Fatty Acid Desaturases
• Stearoyl-CoA Desaturase
• FADS1 protein, human
• FADS2 protein, human

Topics

• Cell Line
• Culture Media
• Delta-5 Fatty Acid Desaturase
• Fatty Acid Desaturases (genetics, metabolism)
• Humans
• Insulin (pharmacology)
• Monocytes (drug effects, metabolism)
• Stearoyl-CoA Desaturase (genetics, metabolism)
• Sterol Regulatory Element Binding Protein 1 (genetics, metabolism)
• Transcription, Genetic
• Up-Regulation