Application of a peptide-mediated magnetic separation-phage assay for detection of viable Mycobacterium avium subsp. paratuberculosis to bovine bulk tank milk and feces samples.

Abstract

Naturally contaminated bovine bulk tank milk (n = 44) and feces (n = 39) were tested for the presence of viable Mycobacterium avium subsp. paratuberculosis by a novel peptide-mediated magnetic separation-phage (PMS-phage) assay. Counts of viable M. avium subsp. paratuberculosis cells ranging from 1 to 110 PFU/50 ml of milk and 6 to 41,111 PFU/g of feces were indicated by the PMS-phage assay.

• Authors: Antonio Foddai, Samuel Strain, Robert H Whitlock, Christopher T Elliott, Irene R Grant
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 49 Issue 5 Pg. 2017-9 (May 2011) ISSN: 1098-660X [Electronic] United States
• PMID: 21411581 (Publication Type: Evaluation Study, Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Bacterial Load
• Bacteriological Techniques (methods)
• Bacteriophages (growth & development)
• Cattle
• Feces (microbiology)
• Microbial Viability
• Milk (microbiology)
• Mycobacterium avium subsp. paratuberculosis (isolation & purification)
• Paratuberculosis (diagnosis, microbiology)
• Sensitivity and Specificity