The effects of oleuropein aglycone, an olive oil compound, in a mouse model of carrageenan-induced pleurisy.

Abstract

BACKGROUND & AIMS:

Several olive oil phenolic compounds, such us oleuropein have attracted considerable attention because of their antioxidant activity, anti-atherosclerotic and anti-inflammatory properties. The aim of this study was to investigate the effects of oleuropein aglycone, a hydrolysis product of oleuropein, in a mouse model of carrageenan-induced pleurisy.

METHODS:

Mice were anaesthetized and subjected to a skin incision at the level of the left sixth intercostals space. The underlying muscle was dissected and saline or saline containing 2% λ-carrageenan was injected into the pleural cavity.

RESULTS:

Injection of carrageenan elicited an acute inflammatory response characterized by: infiltration of neutrophils in lung tissues (P < 0.01 versus sham. P < 0.01 versus carrageenan) and subsequent lipid peroxidation (P < 0.01 versus sham. P < 0.01 versus carrageenan), increased production of tumor necrosis factor-α and interleukin-1β (P < 0.01 versus sham. P < 0.01 versus carrageenan), increased expression of adhesion molecules, increased synthesis of nitric oxide (P < 0.01 versus sham. P < 0.01 versus carrageenan), nitrotyrosine and poly-ADP-ribose (P < 0.01 versus sham. P < 0.01 versus carrageenan). Administration of oleuropein aglycone 30 min after the challenge with carrageenan, caused a significant reduction of all the parameters of inflammation measured.

CONCLUSIONS:

Thus, we propose that olive oil phenolic constituents may be useful in the treatment of various inflammatory diseases.

• Authors: Daniela Impellizzeri, Emanuela Esposito, Emanuela Mazzon, Irene Paterniti, Rosanna Di Paola, Placido Bramanti, Valeria Maria Morittu, Antonio Procopio, Domenico Britti, Salvatore Cuzzocrea
• Journal: Clinical nutrition (Edinburgh, Scotland) (Clin Nutr) Vol. 30 Issue 4 Pg. 533-40 (Aug 2011) ISSN: 1532-1983 [Electronic] England
• PMID: 21411195 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism. All rights reserved.

Chemical References

• Anti-Inflammatory Agents
• Antioxidants
• Interleukin-1beta
• Iridoid Glucosides
• Iridoids
• Olive Oil
• P-Selectin
• Phenols
• Plant Oils
• Pyrans
• Tumor Necrosis Factor-alpha
• Intercellular Adhesion Molecule-1
• Poly Adenosine Diphosphate Ribose
• oleuropein
• Nitric Oxide
• 3-nitrotyrosine
• Tyrosine
• Carrageenan

Topics

• Animals
• Anti-Inflammatory Agents (pharmacology)
• Antioxidants (pharmacology)
• Carrageenan (adverse effects)
• Disease Models, Animal
• Hydrolysis
• Intercellular Adhesion Molecule-1 (drug effects, metabolism)
• Interleukin-1beta (metabolism)
• Iridoid Glucosides
• Iridoids
• Lipid Peroxidation (drug effects)
• Lung (drug effects)
• Male
• Mice
• Nitric Oxide (metabolism)
• Olive Oil
• P-Selectin (metabolism)
• Phenols (pharmacology)
• Plant Oils (chemistry)
• Pleurisy (chemically induced)
• Poly Adenosine Diphosphate Ribose (metabolism)
• Pyrans (pharmacology)
• Tumor Necrosis Factor-alpha (metabolism)
• Tyrosine (analogs & derivatives, metabolism)