Photoinduced nitric oxide release from a nitrobenzene derivative in mitochondria.

Abstract

We report a novel NO donor (RpNO), containing a 2,6-dimethylnitrobenzene moiety for photocontrollable NO release and a rhodamine moiety for targeting to mitochondria. Photorelease of NO from RpNO in aqueous solution was confirmed by means of ESR analysis. Cellular release of NO from RpNO was confirmed with the aid of DAF-FM DA, an NO-specific fluorescence probe. RpNO was colocalized with MitoTracker Green FM, a mitochondrial stain, in HCT116 colon cancer cells and exhibited photodependent cytotoxicity. Our results indicate that RpNO is an effective NO donor for time-controlled, mitochondria-specific NO treatment.

• Authors: Taeko Horinouchi, Hidehiko Nakagawa, Takayoshi Suzuki, Kiyoshi Fukuhara, Naoki Miyata
• Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (Chemistry) Vol. 17 Issue 17 Pg. 4809-13 (Apr 18 2011) ISSN: 1521-3765 [Electronic] Germany
• PMID: 21404338 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Chemical References

• 1-iodo-3,5-dimethylbenzene
• 2,6-dimethylnitrobenzene
• Fluoresceins
• Fluorescent Dyes
• Nitrobenzenes
• Nitric Oxide

Topics

• Cell Line, Tumor
• Colonic Neoplasms (chemistry, drug therapy)
• Fluoresceins (chemistry)
• Fluorescent Dyes (chemistry)
• Humans
• Mitochondria (chemistry, radiation effects)
• Molecular Structure
• Nitric Oxide (chemistry, pharmacology)
• Nitrobenzenes (chemistry)