Kinase suppressor of Ras 2 is involved in regulation of cell proliferation and is up-regulated in human invasive ductal carcinomas of breast.

Abstract

AIM:

To study the expression of Kinase Suppressor of Ras 2 (KSR2) in human breast tumors and its effect on proliferation of breast epithelial cells. We reported previously that KSR2 was up-regulated in immortalized human breast epithelial cells.

METHODS:

Proteomics technologies, systems biology tool for a KSR2 network analysis, immunoblotting, siRNA technology, overexpression of KSR2, cell proliferation assays and immunohistochemistry of tissue microarray of human breast tumors and normal breast tissue were used.

RESULTS:

In conditionally immortalized primary epithelial cells KSR2 expression was shown to be up-regulated. The involvement of KSR2 in regulation of cell proliferation was predicted by a KSR2-centered network analysis. We observed that KSR2 down-regulation with specific siRNA inhibited cell proliferation. By immunohistochemistry of tissue microarray it was demonstrated that KSR2 expression was enhanced in human invasive breast carcinomas.

CONCLUSION:

Our findings propose KSR2 as a new marker of immortalization, which has an impact on cell proliferation.

• Authors: M Jia, S Souchelnytskyi
• Journal: Experimental oncology (Exp Oncol) Vol. 32 Issue 3 Pg. 209-12 (Sep 2010) ISSN: 1812-9269 [Print] Ukraine
• PMID: 21403620 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• KSR2 protein, human
• Protein Serine-Threonine Kinases

Topics

• Breast (metabolism, pathology)
• Breast Neoplasms (genetics, metabolism, pathology)
• Carcinoma, Ductal, Breast (genetics, metabolism, pathology)
• Cell Line, Tumor
• Cell Proliferation
• Epithelial Cells (metabolism, pathology)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Protein Serine-Threonine Kinases (genetics, metabolism)
• Up-Regulation