COS-1 cells as packaging host for production of lentiviruses.

We present a protocol for in vitro production of recombinant lentiviruses using COS-1 African green monkey kidney epithelial cells and HEK293T human embryonic kidney epithelial cells as packaging cells. COS-1 and HEK293T express SV40 large T antigen, amplifying transfected circular plasmids harboring SV40 replication origin. Support protocols for evaluation of transfection efficiency by in situ β-galactosidase enzyme activity assay and titer of infection-capable virions are also provided. Advantages of using COS-1 packaging cells over the standard HEK293T cells for contamination-sensitive applications or automated processing are discussed.
AuthorsCrystal J MacKenzie, Toshi Shioda
JournalCurrent protocols in cell biology / editorial board, Juan S. Bonifacino ... [et al.] (Curr Protoc Cell Biol) Vol. Chapter 26 Pg. Unit 26.7 (Mar 2011) ISSN: 1934-2616 [Electronic] United States
PMID21400698 (Publication Type: Journal Article)
Chemical References
  • Antigens, Polyomavirus Transforming
  • Animals
  • Antigens, Polyomavirus Transforming (genetics)
  • COS Cells
  • Cell Line
  • Cercopithecus aethiops
  • Lentivirus (genetics)
  • Virology (methods)
  • Virus Cultivation (methods)

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