Intraradial administration of fasudil inhibits augmented Rho kinase activity to effectively dilate the spastic radial artery during coronary artery bypass grafting surgery.

Abstract

OBJECTIVES:

Radial arteries are increasingly used as conduits for coronary artery bypass grafts. However, vasospasm continues to be a major concern in radial artery grafts. Rho kinase plays a critical role in vascular contraction through phosphorylation of the regulatory subunit myosin phosphatase targeting subunit 1 (MYPT1) of the myosin light chain phosphatase to inhibit myosin light chain phosphatase in vascular smooth muscle. The purpose of this study was to evaluate the inhibitory effects of fasudil, a clinically used Rho kinase inhibitor, on Rho kinase activity, myosin light chain phosphorylation, in vitro contraction, and in situ vasospasm in radial arteries of patients undergoing coronary artery bypass grafting surgery.

METHODS:

The inhibitory efficacy of fasudil on vasoconstrictor-induced contraction and phosphorylation of MYPT1 was examined in radial artery rings. In situ phosphorylation of MYPT1 was evaluated in nonspastic and spastic radial arteries, and the effects of intraluminal administration of fasudil and verapamil-glyceryl trinitrate (VG) on in situ free blood flow and phosphorylation of MYPT1 and myosin light chain were compared in spastic radial arteries.

RESULTS:

Both fasudil and VG nearly fully inhibited noradrenaline- and serotonin-induced contraction of radial artery rings. However, fasudil but not VG abolished MYPT1 phosphorylation. In spastic radial arteries phosphorylation of MYPT1 and myosin light chain was increased compared with that seen in nonspastic arteries. Intraradial administration of fasudil induced a much larger increase in in situ free blood flow compared with VG treatment. This antispastic effect of fasudil was accompanied by marked decreases in phosphorylation of MYPT1 and myosin light chain.

CONCLUSIONS:

Fasudil is a very effective Rho kinase inhibitor that deinhibits myosin light chain phosphatase and powerfully relieves vasospasm in situ in radial arteries.

• Authors: Takeshi Takagi, Yasuo Okamoto, Shigeyuki Tomita, Atsushi Sato, Shohjiro Yamaguchi, Yoh Takuwa, Go Watanabe
• Journal: The Journal of thoracic and cardiovascular surgery (J Thorac Cardiovasc Surg) Vol. 142 Issue 2 Pg. e59-65 (Aug 2011) ISSN: 1097-685X [Electronic] United States
• PMID: 21397262 (Publication Type: Journal Article)
• Copyright: Copyright © 2011 The American Association for Thoracic Surgery. Published by Mosby, Inc. All rights reserved.

Chemical References

• Myosin Light Chains
• Protein Kinase Inhibitors
• 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine
• rho-Associated Kinases
• Myosin-Light-Chain Phosphatase
• PPP1R12A protein, human
• fasudil

Topics

• 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine (administration & dosage, analogs & derivatives, pharmacology)
• Aged
• Coronary Artery Bypass
• Female
• Humans
• Injections, Intra-Arterial
• Male
• Myosin Light Chains (metabolism)
• Myosin-Light-Chain Phosphatase (antagonists & inhibitors, metabolism)
• Phosphorylation (drug effects)
• Protein Kinase Inhibitors (administration & dosage, pharmacology)
• Radial Artery (drug effects, transplantation)
• Transplantation, Autologous
• Vasodilation
• rho-Associated Kinases (antagonists & inhibitors, metabolism)