[Impact of RelA antisense oligonucleotides on laryngeal carcinoma Hep-2 cell proliferation].

Abstract

OBJECTIVE:

To study the impacts of RelA antisense oligonucleotides on proliferation in laryngeal carcinoma Hep-2 cell.

METHOD:

RelA antisense oligonucleotides was designed, which was transferred into laryngeal carcinoma Hep-2 cell. MTT was used to detect the growth-inhibiting ratio at different transferred timepoints. Hep-2 cell which was transferred 48 h was used to do colony assay, and expression of RelA was detected by Reverse Transcription PCR and Western blot.

RESULT:

MTT results showed that RelA antisense oligonucleotides could significantly suppress the proliferation of Hep-2 cell, and the suppression-ratio elevated with time. There were statistical difference compared with control groups. The number of cells colony was reduced in RelA antisense oligonucleotides group compared with control groups, which had statistic significance. RT-PCR and Western blot results demonstrated that RelA antisense oligonucleotides could significantly inhibit the expression of messenger RNA and protein in Hep-2 cell.

CONCLUSION:

RelA antisense oligonucleotides can inhibit the expression of messenger RNA and protein, and induce the cell proliferation and increase the number of cells colony in Hep-2 cell.

• Authors: Song Pan, Jingzhi Wan, Lilian Wu, Ji Zhao
• Journal: Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery (Lin Chung Er Bi Yan Hou Tou Jing Wai Ke Za Zhi) Vol. 24 Issue 24 Pg. 1135-7 (Dec 2010) ISSN: 2096-7993 [Print] China
• PMID: 21395185 (Publication Type: English Abstract, Journal Article)

Chemical References

• Oligonucleotides, Antisense
• RELA protein, human
• Transcription Factor RelA

Topics

• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Humans
• Laryngeal Neoplasms (genetics, pathology)
• Oligonucleotides, Antisense (genetics, pharmacology)
• Transcription Factor RelA (genetics)