Abstract |
Clinically obtained human kidney stones of different pathogenesis were dissolved in acetic acid/ methanol solutions and then rapidly analyzed by surface desorption atmospheric pressure chemical ionization mass spectrometry (SDAPCI-MS) without any desalination treatment. The mass spectral fingerprints of six groups of kidney stone samples were rapidly recorded in the mass range of m/z 50-400. A set of ten melamine-induced kidney stone samples and nine uric acid derived kidney stone samples were successfully differentiated from other groups by principal component analysis of SDAPCI-MS fingerprints upon positive-ion detection mode. In contrast, the mass spectra recorded using negative-ion detection mode did not give enough information to differentiate those stone samples. The results showed that in addition to the melamine, the chemical compounds enwrapped in the melamine-induced kidney stone samples differed from other kidney stone samples, providing useful hints for studying on the formation mechanisms of melamine-induced kidney stones. This study also provides useful information on establishing a MS-based platform for rapid analysis of the melamine-induced human kidney stones at molecular levels.
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Authors | Bin Jia, Yongzhong Ouyang, Rana N S Sodhi, Bin Hu, Tingting Zhang, Jianqiang Li, Huanwen Chen |
Journal | Journal of mass spectrometry : JMS
(J Mass Spectrom)
Vol. 46
Issue 3
Pg. 313-9
(Mar 2011)
ISSN: 1096-9888 [Electronic] England |
PMID | 21394847
(Publication Type: Journal Article)
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Copyright | Copyright © 2011 John Wiley & Sons, Ltd. |
Chemical References |
- Triazines
- Uric Acid
- melamine
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Topics |
- Adult
- Child
- Child, Preschool
- Female
- Humans
- Kidney Calculi
(chemically induced, chemistry, classification, metabolism)
- Male
- Mass Spectrometry
(methods)
- Middle Aged
- Molecular Imaging
- Principal Component Analysis
- Triazines
(chemistry, poisoning)
- Uric Acid
(chemistry)
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