The effect of N-methyl-D-aspartate receptor antagonists on D,L-homocysteine thiolactone induced seizures in adult rats.

Abstract

The aim of our study was to investigate the effects of ifenprodil and MK-801 on D,L-homocysteine thiolactone induced seizures in adult rats.Male Wistar rats were divided into following groups: 1. Saline-treated (C, n=10); 2. D,L-homocysteine thiolactone 8 mmol/kg, i.p. (H, n=7); 3. Ifenprodil 20 mg/kg i.p. (IF, n=8); 4. MK-801 0.5 mg/kg, i.p. (MK, n=8) and 5. Groups that received IF or MK 30 minutes prior to H (IFH, n=8 and MKH, n=8). Seizure behavior was assessed by incidence, latency, number and intensity of seizure episodes. Seizure severity was described by a descriptive scale with grades 0-4. Lethality in experimental group was recorded 90 min and 24 h upon D,L-homocysteine thiolactone administration.There were no behavioral signs of seizure activity in groups C, IF and MK.Pre-treatment with MK-801 (MKH) showed tendency to reduced incidence of convulsions, latency to the first seizure onset and the severity of seizure episodes, but statistical significance was not attained comparing to the H group. However, median number of seizure episodes was significantly decreased in MKH (p<0.05), comparing to the H group. On the other hand, ifenprodil (IFH) decreased the latency to the first seizure onset and increased the median number of seizure episodes (p<0.05). The majority of seizure episodes in IFH (72.1%, p<0.05) and MKH (73.1%, p<0.05) groups was grade 2 and significantly different comparing to the H (36.0%). Our findings suggest that MK-801 has a mild anticonvulsive effect on D,L-homocysteine thiolactone induced seizures in adult rats.

• Authors: A Rašić-Marković, D Hrnčić, D Djurić, D Macut, H Lončar-Stevanović, Olivera Stanojlović
• Journal: Acta physiologica Hungarica (Acta Physiol Hung) Vol. 98 Issue 1 Pg. 17-26 (Mar 2011) ISSN: 0231-424X [Print] Hungary
• PMID: 21388927 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Piperidines
• Receptors, N-Methyl-D-Aspartate
• Homocysteine
• Dizocilpine Maleate
• homocysteine thiolactone
• ifenprodil

Topics

• Animals
• Dizocilpine Maleate (pharmacology)
• Homocysteine (analogs & derivatives, pharmacology)
• Male
• Piperidines (pharmacology)
• Rats
• Rats, Wistar
• Receptors, N-Methyl-D-Aspartate (antagonists & inhibitors)
• Seizures (chemically induced, drug therapy)