Abstract |
From the 70% ethanol extract of the rhizomes of Cyperus rotundus (CRE), several major constituents including the sesquiterpene derivatives ( valencene, nootkatone, and caryophyllene α- oxide), monoterpenes (β-pinene, 1,8-cineole, and limonene) and 4-cymene were isolated and examined for their anti-allergic activity in vitro and in vivo. In rat basophilic leukemia (RBL)-1 cells, the sesquiterpenes strongly inhibited 5-lipoxygenase-catalyzed leukotrienes production. In addition, they inhibited β- hexosaminidase release by antigen-stimulated RBL-2H3 cells, with valencene having the highest inhibitory effect. CRE inhibited leukotrienes production and β- hexosaminidase release at 300 μg/mL. It was also found that the most active sesquiterpene ( valencene) and CRE inhibited β- hexosaminidase degranulation by inhibiting the initial activation reaction, Lyn phosphorylation, in IgE-stimulated RBL-2H3 cells. Moreover, CRE, valencene and nootkatone significantly inhibited the delayed-type hypersensitivity reaction in mice when administered orally at 50-300 mg/kg. In conclusion, C. rotundus and its constituents, valencene, nootkatone, and caryophyllene α- oxide, exert anti-allergic activity in vitro and in vivo. These sesquiterpenes, but not monoterpenes, certainly contribute to the anti-allergic activity of the rhizomes of C. rotundus.
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Authors | Jeong Ho Jin, Dong-Ung Lee, Yeong Shik Kim, Hyun Pyo Kim |
Journal | Archives of pharmacal research
(Arch Pharm Res)
Vol. 34
Issue 2
Pg. 223-8
(Feb 2011)
ISSN: 1976-3786 [Electronic] Korea (South) |
PMID | 21380805
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Allergic Agents
- Plant Extracts
- Sesquiterpenes
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Topics |
- Animals
- Anti-Allergic Agents
(chemistry, isolation & purification, pharmacology)
- Cell Degranulation
(drug effects)
- Cyperus
- Drug Screening Assays, Antitumor
- Hypersensitivity, Delayed
(drug therapy)
- Male
- Mice
- Mice, Inbred ICR
- Phytotherapy
- Plant Extracts
(chemistry, isolation & purification, pharmacology)
- Rats
- Rhizome
- Sesquiterpenes
(chemistry, isolation & purification, pharmacology)
- Tumor Cells, Cultured
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