Opisthorchiasis-associated
cholangiocarcinoma (CCA) is one of main public health problems in Opisthorchis viverrini endemic areas. Although the definite relationship between prevalence of CCA and the
parasite infection has been demonstrated, the molecular mechanism of
tumorigenesis is still unknown. In the present study, by using animal model of
opisthorchiasis-associated CCA, a kinetic analysis of
cDNA microarray was performed to screen the candidate genes that involve in the development of
opisthorchiasis-associated CCA. Microarray analysis revealed that the expressions of 131 genes were up-regulated during the development of CCA, including the genes relative to cell proliferation, differentiation and transformation, cell growth and cycle regulation, apoptosis, DNA repair, and cytoskeletal structure. The expressions of 145 genes were down-regulated, including the genes relative to metabolic
enzymes,
tumor suppressor, apoptosis, and oxidative response and oxidation reduction. The present study listed up the candidate genes involving
tumorigenesis, provided molecular information on the development of
opisthorchiasis-associated CCA and the potential
biomarkers for diagnosis and
therapy, and suggested that the increased expression of cell differentiation, proliferation, transformation-related genes, and decreased expression of metabolic
enzymes may play important roles in the
tumorigenesis of CCA.