Addition of 2-deoxyglucose enhances growth inhibition but reverses acidification in colon cancer cells treated with phenformin.
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| Abstract |
A report that effects of butyrate on some cells may be mediated by activation of AMP-activated protein kinase (AMPK) prompted this study which examines if other AMPK activators can induce differentiation and inhibit proliferation of colon cancer cells in a manner similar to butyrate. Using induction of alkaline phosphatase as a marker, it was observed that compound C, an AMPK inhibitor, is able to reduce the differentiating effect of butyrate on SW1116 and Caco-2 colon cancer cells. Metformin was observed to be less effective than butyrate in the induction of alkaline phosphatase but was more effective as a growth inhibitor. Phenformin was found to be a more potent growth inhibitor than metformin and both compounds cause acidification of the medium when incubated with colon cancer cells. Combined incubation of 2-deoxyglucose with either of the biguanides prevented the acidification of the medium but enhanced the growth inhibitory effects.
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| Authors | Michael A Lea, Jerel Chacko, Sandhya Bolikal, Ji Y Hong, Ryan Chung, Andres Ortega, Charles desbordes |
| Journal | Anticancer research (Anticancer Res) Vol. 31 Issue 2 Pg. 421-6 (Feb 2011) ISSN: 1791-7530 [Electronic] Greece |
| PMID | 21378320 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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