Abstract | BACKGROUND: METHODS: Mother rats fed a soybean-based, docosahexaenoic acid (DHA)- or eicosapentaenoic acid (EPA)-enriched diet from days 7 to 20 of gestation were examined. On day 20, the rat pups were delivered by abdominal incision, their intestines were removed, and messenger RNA was extracted. A rat NEC model was used to confirm the effects of ω-3 fatty acids on the inflamed intestine (n = 20-28). The expression of inflammatory molecules was analyzed by real-time polymerase chain reaction (n = 11-14). RESULTS: The concentrations of DHA and EPA in the intestine were significantly increased in the DHA and EPA groups (P < .01). The expression of the antiinflammatory prostaglandin E2 receptor EP3 was increased in the DHA (P < .05) and EPA groups (P < .01). In the NEC model, the reduced incidence of colitis was confirmed in the DHA and EPA groups. The expression of peroxisome proliferator-activated receptor γ was increased (P < .05), and the inhibitor of nuclear factor-κB α/β decreased in both the DHA (P < .01) and EPA groups (P < .05). CONCLUSION: Our findings indicate that ω-3 fatty acids are beneficial for protecting the premature intestine from inflammation by regulating eicosanoid- and nuclear factor-κB-related metabolite expression.
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Authors | Yoshikazu Ohtsuka, Kyo Okada, Yoko Yamakawa, Tamaki Ikuse, Yosuke Baba, Eisuke Inage, Tohru Fujii, Hirohisa Izumi, Kyoichi Oshida, Satoru Nagata, Yuichiro Yamashiro, Toshiaki Shimizu |
Journal | Journal of pediatric surgery
(J Pediatr Surg)
Vol. 46
Issue 3
Pg. 489-95
(Mar 2011)
ISSN: 1531-5037 [Electronic] United States |
PMID | 21376198
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Inc. All rights reserved. |
Chemical References |
- Anti-Inflammatory Agents
- Dietary Fats, Unsaturated
- Fatty Acids
- NF-kappa B
- PPAR gamma
- Receptors, Prostaglandin E, EP3 Subtype
- Docosahexaenoic Acids
- Soybean Oil
- Eicosapentaenoic Acid
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Topics |
- Animals
- Animals, Newborn
- Anti-Inflammatory Agents
(administration & dosage, pharmacology, therapeutic use)
- Dietary Fats, Unsaturated
(administration & dosage, therapeutic use)
- Disease Models, Animal
- Docosahexaenoic Acids
(administration & dosage, pharmacology, therapeutic use)
- Drug Evaluation, Preclinical
- Eicosapentaenoic Acid
(administration & dosage, pharmacology, therapeutic use)
- Enterocolitis, Necrotizing
(chemically induced, prevention & control)
- Fatty Acids
(analysis)
- Female
- Gene Expression Regulation
(drug effects)
- Ileum
(chemistry, drug effects, embryology)
- Infant Food
(toxicity)
- Intestinal Mucosa
(drug effects)
- Maternal-Fetal Exchange
- Models, Animal
- NF-kappa B
(drug effects)
- PPAR gamma
(biosynthesis, genetics)
- Pregnancy
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Receptors, Prostaglandin E, EP3 Subtype
(biosynthesis, genetics)
- Soybean Oil
- Specific Pathogen-Free Organisms
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