Abstract |
Lentivectors are potential vaccine delivery vehicles because they can efficiently transduce a variety of non-dividing cells, including antigen-presenting cells, and do not cause expression of extra viral proteins. To improve safety while retaining efficiency, a dendritic cell (DC)-specific lentivector was constructed by pseudotyping the vector with an engineered viral glycoprotein derived from Sindbis virus. We assessed the level of anti- tumor immunity conferred by this engineered lentivector encoding the melanoma antigen gp100 in a mouse model. Footpad injection of the engineered lentivectors results in the best antigen-specific immune response as compared with subcutaneous and intraperitoneal injections. A single prime vaccination of the engineered lentivectors can elicit a high frequency (up to 10%) of gp100-specific CD8(+) T cells in peripheral blood 3 weeks after the vaccination and this response will be maintained at around 5% for up to 8 weeks. We found that these engineered lentivectors elicited relatively low levels of anti-vector neutralizing antibody responses. Importantly, direct injection of this engineered lentivector inhibited the growth of aggressive B16 murine melanoma. These data suggest that DC-specific lentivectors can be a novel and alternative vaccine carrier with the potential to deliver effective anti- tumor immunity for cancer immunotherapy.
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Authors | H G Yang, B L Hu, L Xiao, P Wang |
Journal | Cancer gene therapy
(Cancer Gene Ther)
Vol. 18
Issue 5
Pg. 370-80
(May 2011)
ISSN: 1476-5500 [Electronic] England |
PMID | 21372855
(Publication Type: Journal Article, Research Support, N.I.H., Extramural)
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Copyright | © 2011 Nature America, Inc. |
Chemical References |
- Cancer Vaccines
- Cytokines
- DNA Primers
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Topics |
- Animals
- CD8-Positive T-Lymphocytes
(immunology)
- Cancer Vaccines
(administration & dosage, immunology)
- Cytokines
(immunology)
- DNA Primers
(genetics)
- Dendritic Cells
(metabolism, virology)
- Enzyme-Linked Immunospot Assay
- Female
- Flow Cytometry
- Genetic Therapy
(methods)
- Genetic Vectors
(administration & dosage, immunology)
- Immunotherapy
(methods)
- Lentivirus
- Melanoma
(genetics, immunology, therapy)
- Mice
- Mice, Inbred C57BL
- Neutralization Tests
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