Topical application of aloperine improves 2,4-dinitrofluorobenzene-induced atopic dermatitis-like skin lesions in NC/Nga mice.

Abstract

Aloperine has been shown to inhibit 2,4-dinitrofluorobenzene (DNFB) induced allergic contact dermatitis in BALB/c mice. In the present study, we further investigated the effect of aloperine on DNFB-induced atopic dermatitis-like skin lesions in NC/Nga mice. NC/Nga mice elicited atopic dermatitis-like skin lesions after the topical application of DNFB. Aloperine treatment significantly inhibited dermatitis index and ear thickness in DNFB-treated NC/Nga mice in a dose-dependent manner. Eosinophils, mast cells infiltration into the ears and plasma level of immunoglobulin (Ig) E were also suppressed by aloperine treatment. Finally, cytokine (interleukin (IL)-1β, IL-4, IL-6, IL-10, IL-13, tumor necrosis factor (TNF)-α and interferon (IFN)-γ) productions in ear biopsies homogenates were significantly elevated after DNFB challenge. Topical application of aloperine increased the immunosuppressive cytokine IL-10 level, while it reduced other cytokines production in a dose-dependent manner. Taken together, these data suggest that aloperine may be one of the effective therapeutic agents for the treatment of atopic dermatitis.

• Authors: Xiao-Ying Yuan, Hui-Min Ma, Rui-Zhi Li, Rui-Yan Wang, Wei Liu, Jian-You Guo
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 658 Issue 2-3 Pg. 263-9 (May 11 2011) ISSN: 1879-0712 [Electronic] Netherlands
• PMID: 21371468 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Piperidines
• Quinolizidines
• Interleukin-10
• Immunoglobulin E
• aloperine
• Dinitrofluorobenzene

Topics

• Administration, Topical
• Animals
• Dermatitis, Atopic (chemically induced, drug therapy, immunology, metabolism)
• Dinitrofluorobenzene (pharmacology)
• Down-Regulation (drug effects)
• Ear (pathology)
• Female
• Immunoglobulin E (blood)
• Interleukin-10 (metabolism)
• Mast Cells (drug effects, immunology)
• Mice
• Piperidines (administration & dosage, pharmacology, therapeutic use)
• Quinolizidines
• Th1 Cells (drug effects, immunology)
• Th2 Cells (drug effects, immunology)
• Up-Regulation (drug effects)