Abstract |
Dendritic cell (DC)-based immunotherapy is a potent therapeutic modality for treating renal cell carcinoma (RCC), but development of antigens specific for tumor-targeting and anti- tumor immunity is of great interest for clinical trials. The present study investigated the ability of DCs pulsed with a combination of carbonic anhydrase IX (CA9) as an RCC-specific biomarker and Acinetobacter baumannii outer membrane protein A (AbOmpA) as an immunoadjuvant to induce anti- tumor immunity against murine renal cell carcinoma (RENCA) in a murine model. Murine bone-marrow-derived DCs pulsed with a combination of RENCA lysates and AbOmpA were tested for their capacity to induce DC maturation and T cell responses in vitro. A combination of RENCA lysates and AbOmpA up-regulated the surface expression of co-stimulatory molecules, CD80 and CD86, and the antigen presenting molecules, major histocompatibility (MHC) class I and class II, in DCs. A combination of RENCA lysates and AbOmpA also induced interleukin-12 (IL-12) production in DCs. Next, the immunostimulatory activity of DCs pulsed with a combination of CA9 and AbOmpA was determined. A combination of CA9 and AbOmpA up-regulated the surface expression of co-stimulatory molecules and antigen presenting molecules in DCs. DCs pulsed with a combination of CA9 and AbOmpA effectively secreted IL-12 but not IL-10. These cells interacted with T cells and formed clusters. DCs pulsed with CA9 and AbOmpA elicited the secretion of interferon-γ and IL-2 in T cells. In conclusion, a combination of CA9 and AbOmpA enhanced the immunostimulatory activity of DCs, which may effectively induce anti- tumor immunity against human RCC.
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Authors | Bo Ra Kim, Eun Kyoung Yang, Sun Hee Kim, Dong Chan Moon, Hwa Jung Kim, Je Chul Lee, Duk Yoon Kim |
Journal | Journal of microbiology (Seoul, Korea)
(J Microbiol)
Vol. 49
Issue 1
Pg. 115-20
(Feb 2011)
ISSN: 1976-3794 [Electronic] Korea (South) |
PMID | 21369988
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Neoplasm
- B7-1 Antigen
- B7-2 Antigen
- Bacterial Outer Membrane Proteins
- Cancer Vaccines
- Histocompatibility Antigens Class I
- Histocompatibility Antigens Class II
- Interleukin-10
- OMPA outer membrane proteins
- Interleukin-12
- CA9 protein, human
- Carbonic Anhydrase IX
- Carbonic Anhydrases
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Topics |
- Acinetobacter baumannii
(immunology)
- Animals
- Antigens, Neoplasm
(genetics, immunology)
- B7-1 Antigen
(analysis)
- B7-2 Antigen
(analysis)
- Bacterial Outer Membrane Proteins
(immunology)
- Cancer Vaccines
(immunology)
- Carbonic Anhydrase IX
- Carbonic Anhydrases
(genetics, immunology)
- Carcinoma, Renal Cell
(therapy)
- Dendritic Cells
(chemistry, immunology)
- Disease Models, Animal
- Gene Expression Profiling
- Histocompatibility Antigens Class I
(analysis)
- Histocompatibility Antigens Class II
(analysis)
- Humans
- Immunotherapy
(methods)
- Interleukin-10
(metabolism)
- Interleukin-12
(metabolism)
- Mice
- Mice, Inbred BALB C
- T-Lymphocytes
(immunology)
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