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Immunostimulatory activity of dendritic cells pulsed with carbonic anhydrase IX and Acinetobacter baumannii outer membrane protein A for renal cell carcinoma.

Abstract
Dendritic cell (DC)-based immunotherapy is a potent therapeutic modality for treating renal cell carcinoma (RCC), but development of antigens specific for tumor-targeting and anti-tumor immunity is of great interest for clinical trials. The present study investigated the ability of DCs pulsed with a combination of carbonic anhydrase IX (CA9) as an RCC-specific biomarker and Acinetobacter baumannii outer membrane protein A (AbOmpA) as an immunoadjuvant to induce anti-tumor immunity against murine renal cell carcinoma (RENCA) in a murine model. Murine bone-marrow-derived DCs pulsed with a combination of RENCA lysates and AbOmpA were tested for their capacity to induce DC maturation and T cell responses in vitro. A combination of RENCA lysates and AbOmpA up-regulated the surface expression of co-stimulatory molecules, CD80 and CD86, and the antigen presenting molecules, major histocompatibility (MHC) class I and class II, in DCs. A combination of RENCA lysates and AbOmpA also induced interleukin-12 (IL-12) production in DCs. Next, the immunostimulatory activity of DCs pulsed with a combination of CA9 and AbOmpA was determined. A combination of CA9 and AbOmpA up-regulated the surface expression of co-stimulatory molecules and antigen presenting molecules in DCs. DCs pulsed with a combination of CA9 and AbOmpA effectively secreted IL-12 but not IL-10. These cells interacted with T cells and formed clusters. DCs pulsed with CA9 and AbOmpA elicited the secretion of interferon-γ and IL-2 in T cells. In conclusion, a combination of CA9 and AbOmpA enhanced the immunostimulatory activity of DCs, which may effectively induce anti-tumor immunity against human RCC.
AuthorsBo Ra Kim, Eun Kyoung Yang, Sun Hee Kim, Dong Chan Moon, Hwa Jung Kim, Je Chul Lee, Duk Yoon Kim
JournalJournal of microbiology (Seoul, Korea) (J Microbiol) Vol. 49 Issue 1 Pg. 115-20 (Feb 2011) ISSN: 1976-3794 [Electronic] Korea (South)
PMID21369988 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Neoplasm
  • B7-1 Antigen
  • B7-2 Antigen
  • Bacterial Outer Membrane Proteins
  • Cancer Vaccines
  • Histocompatibility Antigens Class I
  • Histocompatibility Antigens Class II
  • Interleukin-10
  • OMPA outer membrane proteins
  • Interleukin-12
  • CA9 protein, human
  • Carbonic Anhydrase IX
  • Carbonic Anhydrases
Topics
  • Acinetobacter baumannii (immunology)
  • Animals
  • Antigens, Neoplasm (genetics, immunology)
  • B7-1 Antigen (analysis)
  • B7-2 Antigen (analysis)
  • Bacterial Outer Membrane Proteins (immunology)
  • Cancer Vaccines (immunology)
  • Carbonic Anhydrase IX
  • Carbonic Anhydrases (genetics, immunology)
  • Carcinoma, Renal Cell (therapy)
  • Dendritic Cells (chemistry, immunology)
  • Disease Models, Animal
  • Gene Expression Profiling
  • Histocompatibility Antigens Class I (analysis)
  • Histocompatibility Antigens Class II (analysis)
  • Humans
  • Immunotherapy (methods)
  • Interleukin-10 (metabolism)
  • Interleukin-12 (metabolism)
  • Mice
  • Mice, Inbred BALB C
  • T-Lymphocytes (immunology)

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