Abstract | CONTEXT: OBJECTIVE: To examine the level of expression and phosphorylation of 4E-BP1 in various subtypes of B-cell lymphoma and reactive lymphoid tissues. DESIGN: Archival formalin-fixed, paraffin-embedded B-cell lymphoma samples and reactive lymphoid tissues were immunostained and examined for expression of 4E-BP1 and phosphorylated 4E-BP1. Expression of components of the eIF-4F complex and unphosphorylated and phosphorylated 4E-BP1 was confirmed using Western immunoblotting on lysates of frozen lymphoma samples and reactive tissues. RESULTS: Immunohistochemical analysis demonstrated weak to undetectable 4E-BP1 staining within benign, reactive germinal centers (N = 10). In contrast, 4E-BP1 was consistently expressed (moderate to strong staining) in 98% of various subtypes of mature B-cell lymphoma (N = 50). 4E-BP1 expression was also demonstrable in all 4 lymph nodes with in situ or partial involvement by follicular lymphoma and in all 12 cases of BCL2-negative lymphoma. The level of phosphorylation of 4E-BP1 in lymphomas, evaluated by immunohistochemistry, was heterogeneous. CONCLUSIONS: The immunohistochemical expression pattern of 4E-BP1 exhibits regional and cellular specificity in reactive lymphoid tissues and may offer a diagnostic tool for distinguishing reactive follicles from neoplastic B-cell proliferations.
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Authors | Dhatri Kodali, Ajay Rawal, Mary J Ninan, Manish R Patel, Hector Mesa, Dennis Knapp, Bertram Schnitzer, Robert A Kratzke, Pankaj Gupta |
Journal | Archives of pathology & laboratory medicine
(Arch Pathol Lab Med)
Vol. 135
Issue 3
Pg. 365-71
(Mar 2011)
ISSN: 1543-2165 [Electronic] United States |
PMID | 21366462
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Adaptor Proteins, Signal Transducing
- Biomarkers, Tumor
- Cell Cycle Proteins
- EIF4EBP1 protein, human
- Phosphoproteins
- RNA Caps
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Topics |
- Adaptor Proteins, Signal Transducing
(genetics, metabolism)
- Biomarkers, Tumor
(metabolism)
- Blotting, Western
- Cell Cycle Proteins
- Germinal Center
(metabolism, pathology)
- Humans
- Immunohistochemistry
- Lymph Nodes
(metabolism, pathology)
- Lymphoid Tissue
(metabolism, pathology)
- Lymphoma, B-Cell
(genetics, metabolism, pathology)
- Lymphoma, Follicular
(metabolism, pathology)
- Phosphoproteins
(genetics, metabolism)
- Phosphorylation
- Pseudolymphoma
(genetics, metabolism, pathology)
- RNA Caps
(metabolism)
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