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Expression and phosphorylation of eukaryotic translation initiation factor 4E binding protein 1 in B-cell lymphomas and reactive lymphoid tissues.

AbstractCONTEXT:
Cap-mediated messenger RNA translation controlled by the eukaryotic initiation factor 4F (eIF-4F) complex plays a key role in human cancer. eIF-4F activity is controlled by a repressor binding protein (4E-BP1), which promotes translation when phosphorylated.
OBJECTIVE:
To examine the level of expression and phosphorylation of 4E-BP1 in various subtypes of B-cell lymphoma and reactive lymphoid tissues.
DESIGN:
Archival formalin-fixed, paraffin-embedded B-cell lymphoma samples and reactive lymphoid tissues were immunostained and examined for expression of 4E-BP1 and phosphorylated 4E-BP1. Expression of components of the eIF-4F complex and unphosphorylated and phosphorylated 4E-BP1 was confirmed using Western immunoblotting on lysates of frozen lymphoma samples and reactive tissues.
RESULTS:
Immunohistochemical analysis demonstrated weak to undetectable 4E-BP1 staining within benign, reactive germinal centers (N = 10). In contrast, 4E-BP1 was consistently expressed (moderate to strong staining) in 98% of various subtypes of mature B-cell lymphoma (N = 50). 4E-BP1 expression was also demonstrable in all 4 lymph nodes with in situ or partial involvement by follicular lymphoma and in all 12 cases of BCL2-negative lymphoma. The level of phosphorylation of 4E-BP1 in lymphomas, evaluated by immunohistochemistry, was heterogeneous.
CONCLUSIONS:
The immunohistochemical expression pattern of 4E-BP1 exhibits regional and cellular specificity in reactive lymphoid tissues and may offer a diagnostic tool for distinguishing reactive follicles from neoplastic B-cell proliferations.
AuthorsDhatri Kodali, Ajay Rawal, Mary J Ninan, Manish R Patel, Hector Mesa, Dennis Knapp, Bertram Schnitzer, Robert A Kratzke, Pankaj Gupta
JournalArchives of pathology & laboratory medicine (Arch Pathol Lab Med) Vol. 135 Issue 3 Pg. 365-71 (Mar 2011) ISSN: 1543-2165 [Electronic] United States
PMID21366462 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Adaptor Proteins, Signal Transducing
  • Biomarkers, Tumor
  • Cell Cycle Proteins
  • EIF4EBP1 protein, human
  • Phosphoproteins
  • RNA Caps
Topics
  • Adaptor Proteins, Signal Transducing (genetics, metabolism)
  • Biomarkers, Tumor (metabolism)
  • Blotting, Western
  • Cell Cycle Proteins
  • Germinal Center (metabolism, pathology)
  • Humans
  • Immunohistochemistry
  • Lymph Nodes (metabolism, pathology)
  • Lymphoid Tissue (metabolism, pathology)
  • Lymphoma, B-Cell (genetics, metabolism, pathology)
  • Lymphoma, Follicular (metabolism, pathology)
  • Phosphoproteins (genetics, metabolism)
  • Phosphorylation
  • Pseudolymphoma (genetics, metabolism, pathology)
  • RNA Caps (metabolism)

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