Abstract | PURPOSE: METHODS: RESULTS: GFP expression was clearly demonstrated, primarily in the retinal pigment epithelium (RPE) and outer retina, 1-6 weeks after delivery. mRNA expression levels of VEGF-B167 and VEGF-B186 were 5.8 and 12 fold higher in the AAV-VEGF-B167- and AAV-VEGF-B186-treated groups, respectively. There was no evidence of an inflammatory response or vessel abnormality following injection of the vectors in normal mice; however, VEGF-B increased retinal and choroidal neovascularization. AAV-VEGF-B186, but not AAV-VEGF-B167, enhanced retinal vascular permeability. CONCLUSIONS:
|
Authors | Xiufeng Zhong, Hu Huang, Jikui Shen, Serena Zacchigna, Lorena Zentilin, Mauro Giacca, Stanley A Vinores |
Journal | Molecular vision
(Mol Vis)
Vol. 17
Pg. 492-507
(Feb 17 2011)
ISSN: 1090-0535 [Electronic] United States |
PMID | 21364963
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Vascular Endothelial Growth Factor B
- Green Fluorescent Proteins
- Oxygen
|
Topics |
- Animals
- Capillary Permeability
(physiology)
- Choroidal Neovascularization
(complications, genetics, physiopathology)
- Dependovirus
(genetics)
- Gene Transfer Techniques
- Genetic Vectors
(genetics)
- Green Fluorescent Proteins
(metabolism)
- Inflammation
(complications, physiopathology)
- Ischemia
(complications, physiopathology)
- Mice
- Mice, Inbred C57BL
- Oxygen
- Recombination, Genetic
(genetics)
- Retina
(pathology, physiopathology)
- Retinal Neovascularization
(complications, genetics, physiopathology)
- Transgenes
(genetics)
- Vascular Endothelial Growth Factor B
(genetics)
|