Abstract |
Artemisinin, a sesquiterpene lactone from Artemisia annua L., has received considerable attention in the last few decades as a potent antimalarial drug. Artemisinin has rather low toxicity; it is effective against drug-resistant Plasmodium species and against cerebral malaria. This study reports the development of a rapid and sensitive assay for the quantification of artemisinin in A. annua by reversed phase HPLC/MS. In the selected optimal experimental conditions, artemisinin exhibited a well-defined chromatographic peak with a retention time of 2 ± 0.2 min. The chromatographic signal shows a linear dependence with artemisinin concentration, enabling the use of this signal for artemisinin quantification according to the following regression equation: y = 2665.40x - 14697.61. The correlation coefficient (R(2)) was 0.9989. For every concentration within the range of the standard curve (0.1-2 µg mL(-1)), accuracy was between 95 and 104%. Artemisinin content in Romanian A. annua wild plants varies between 0.17 and 0.21% (dry weight basis).
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Authors | B Ivanescu, L Vlase, A Corciova, M I Lazar |
Journal | Natural product research
(Nat Prod Res)
Vol. 25
Issue 7
Pg. 716-22
(Apr 2011)
ISSN: 1478-6427 [Electronic] England |
PMID | 21360406
(Publication Type: Journal Article)
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Chemical References |
- Antimalarials
- Artemisinins
- Plant Extracts
- artemisinin
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Topics |
- Antimalarials
(analysis)
- Artemisia annua
(chemistry)
- Artemisinins
(analysis)
- Chromatography, High Pressure Liquid
(methods)
- Plant Components, Aerial
(chemistry)
- Plant Extracts
(chemistry)
- Romania
- Tandem Mass Spectrometry
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