Abstract | BACKGROUND: RESULTS: MRP4 mRNA/ protein levels were markedly increased in obstructive cholestasis. Concentration of serum total bile acids (TBA) was significantly correlated with MRP4 protein in cholestasis samples (P < 0.01). PXR and RXRα mRNA/ protein levels were significantly increased in obstructive cholestasis. CAR mRNA levels were unchanged while protein levels were markedly induced in obstructive cholestasis. There was a statistically positive correlation between MRP4 mRNA and CAR protein (P < 0.05), suggesting that CAR may activate transcription of MRP4 genes by its nuclear translocation. CONCLUSION: Hepatic MRP4 levels were dramatically induced in human obstructive cholestasis, which may reduce liver injury by increasing efflux of toxic bile acids from hepatocytes into blood.
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Authors | Jin Chai, Donglin Luo, Xiaoping Wu, Huaizhi Wang, Yu He, Qiong Li, Yanmei Zhang, Lei Chen, Zhi-Hong Peng, Tianli Xiao, Rongquan Wang, Wensheng Chen |
Journal | Journal of gastrointestinal surgery : official journal of the Society for Surgery of the Alimentary Tract
(J Gastrointest Surg)
Vol. 15
Issue 6
Pg. 996-1004
(Jun 2011)
ISSN: 1873-4626 [Electronic] United States |
PMID | 21359593
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- ABCC4 protein, human
- Bile Acids and Salts
- Constitutive Androstane Receptor
- Multidrug Resistance-Associated Proteins
- Pregnane X Receptor
- RNA, Messenger
- Receptors, Cytoplasmic and Nuclear
- Receptors, Steroid
- Retinoid X Receptor alpha
- Bilirubin
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Topics |
- Bile Acids and Salts
(blood)
- Bilirubin
(blood)
- Blotting, Western
- Cholestasis, Extrahepatic
(metabolism)
- Common Bile Duct
(metabolism)
- Constitutive Androstane Receptor
- Female
- Fluorescent Antibody Technique
- Gene Expression
- Humans
- Liver
(metabolism)
- Male
- Middle Aged
- Multidrug Resistance-Associated Proteins
(genetics, metabolism)
- Pregnane X Receptor
- RNA, Messenger
(metabolism)
- Receptors, Cytoplasmic and Nuclear
(genetics, metabolism)
- Receptors, Steroid
(genetics, metabolism)
- Retinoid X Receptor alpha
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
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