Abstract |
Human papillomaviruses (HPVs) have been linked to a variety of human diseases, most notably cancer of the cervix. In the majority of cases, HPV proteins E6 and E7 are continuously expressed and bind a variety of cellular proteins. The precise mechanism of HPV-induced carcinogenesis has not been fully elucidated; therefore, we attempted to identify the cellular proteins that interact with HPV18 E7 to better understand the function of this important protein. Using yeast 2-hybrid screening, we identified centromere protein C ( CENP-C) as one of the proteins that interact with HPV18 E7. CENP-C interacted with E7 from HPV18 but not from HPV11. The CR2 domain of HPV18 E7 and the C-terminal region of CENP-C were found to be involved in the binding of these proteins. CENP-C is a component of the inner kinetochore and plays an essential role in proper chromosome segregation, mitotic checkpoint function, and kinetochore assembly. HPV18 E7-CENP-C binding may therefore impair centromere function, in turn causing cancers. We speculate that altered function of CENP-C as a result of interactions with HPV E7 may be associated with chromosomal abnormalities in HPV18-positive cancers.
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Authors | Yuji Yaginuma, Kinya Yoda, Katsuhiro Ogawa |
Journal | Oncology
(Oncology)
Vol. 79
Issue 3-4
Pg. 219-28
( 2010)
ISSN: 1423-0232 [Electronic] Switzerland |
PMID | 21358210
(Publication Type: Journal Article)
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Copyright | Copyright © 2011 S. Karger AG, Basel. |
Chemical References |
- Chromosomal Proteins, Non-Histone
- DNA-Binding Proteins
- E7 protein, Human papillomavirus type 18
- Oncogene Proteins, Viral
- centromere protein C
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Topics |
- Brain
(metabolism)
- Chromosomal Proteins, Non-Histone
(genetics, metabolism)
- DNA-Binding Proteins
(genetics, metabolism)
- Fetus
(metabolism)
- Gene Library
- Humans
- Immunoblotting
- Immunoprecipitation
- Oncogene Proteins, Viral
(genetics, metabolism)
- Protein Binding
- Two-Hybrid System Techniques
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