[Protective effect of shenfu injection on kidney against ischemia-reperfusion injury in rats].

Abstract

OBJECTIVE:

To investigate the effect of Shenfu injection on ischemia-reperfusion injury of rat kidney and its mechanism.

METHODS:

Fifty-five male SD rats were randomly divided into 5 groups: sham operation group (Sham group), Ischemia-reperfusion group (IR group), Shenfu injection (5 mL/kg) treated group (SF5 + IR group), Shenfu Injection (10 mL/kg) treated group (SF10+IR group) and Shenfu Injection (15 mL/kg) treated group (SF15+IR group). The rats were anesthetized with valebarbitone. Bilateral kidneys were exposed through midline incision. Right kidney was removed and left renal pedicels were occluded for 50 min with a traumatic mini-clamp then unclamped for 24 h. Animals in (SF5+IR group), (SF10+IR group) and (SF15+IR group) received Shenfu Injection of 5 mL/kg, 10 mL/kg and 15 mL/kg respectively through intraperitoneal injection every day for 7 days. At 24 hours after reperfusion, blood and renal tissue were taken for examination.

RESULTS:

Ischemia-reperfusion injury rat model was established successfully. The level of serum BUN, CREA and MDA were lower in (SF5+IR) group and (SF10+IR)group and (SF15+IR) group than in IR group (P < 0.05). The level of SOD in (SF5+IR) group and (SF10+IR) group and (SF15+IR) group increased significantly than in IR group (P < 0.05). Furthermore, all of the changes were observed in a dose-dependent manner, (SF15 + IR) group demonstrated the best effect by compared with others (P < 0.05).

CONCLUSION:

Shenfu injection can protect rats against acute renal ischemia-reperfusion injury by increasing SOD activity and decreasing MDA content.

• Authors: Yi Li, Ying Wang, Jue Li, Ting-mo Zhang
• Journal: Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition (Sichuan Da Xue Xue Bao Yi Xue Ban) Vol. 42 Issue 1 Pg. 41-3, 77 (Jan 2011) ISSN: 1672-173X [Print] China
• PMID: 21355298 (Publication Type: English Abstract, Journal Article)

Chemical References

• Drugs, Chinese Herbal
• Protective Agents
• Shen-Fu
• Malondialdehyde
• Superoxide Dismutase

Topics

• Animals
• Drugs, Chinese Herbal (pharmacology)
• Injections, Intraperitoneal
• Kidney (blood supply)
• Male
• Malondialdehyde (blood)
• Protective Agents (pharmacology)
• Random Allocation
• Rats
• Rats, Sprague-Dawley
• Reperfusion Injury (metabolism, prevention & control)
• Superoxide Dismutase (metabolism)