HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Intraoperative qRT-PCR for detection of lymph node metastasis in head and neck cancer.

AbstractPURPOSE:
Sentinel node biopsy (SNB) has been shown to accurately stage the regional lymphatics in oral carcinoma. However, intraoperative pathology is only moderately sensitive and final pathology takes several days to complete. The purpose of this study was to develop a rapid, automated, and quantitative real-time PCR (qRT-PCR) assay that can match final pathology in an intraoperative time frame.
EXPERIMENTAL DESIGN:
Four hundred forty-eight grossly tumor-negative lymph nodes were evaluated for expression of 3 markers [PVA (pemphigus vulgaris antigen), PTHrP (parathyroid hormone-related protein), and TACSTD1 (tumor-associated calcium signal transducer 1)]. Conformity of metastasis detection by qRT-PCR was determined using hematoxylin and eosin and immunohistochemistry staining as the gold standard. PVA and TACSTD1 were then multiplexed with β-glucuronidase to develop a rapid, automated single-tube qRT-PCR assay using the Cepheid GeneXpert system. This assay was used to analyze 103 lymph nodes in an intraoperative time frame.
RESULTS:
Four hundred forty-two nodes produced an informative result for both qRT-PCR and pathologic examination. Concordance of qRT-PCR for individual markers with final pathology ranged from 93% to 98%. The best marker combination was TACSTD1 and PVA. A rapid, multiplex assay for TACSTD1 and PVA was developed on the Cepheid GeneXpert and demonstrated an excellent reproducibility and linearity. Analysis of 103 lymph nodes demonstrated 94.2% accuracy of this assay for identifying positive and negative nodes. The average time for each assay to yield results was 35 minutes.
CONCLUSIONS:
A rapid, automated qRT-PCR assay can detect lymph node metastasis in head and neck cancer with high accuracy compared to pathologic analysis and may be more accurate than intraoperative pathology. Combined, SNB and rapid qRT-PCR could more appropriately guide surgical treatment of patients with head and neck cancer.
AuthorsRobert L Ferris, Liqiang Xi, Raja R Seethala, Jon Chan, Shaun Desai, Benjamin Hoch, William Gooding, Tony E Godfrey
JournalClinical cancer research : an official journal of the American Association for Cancer Research (Clin Cancer Res) Vol. 17 Issue 7 Pg. 1858-66 (Apr 01 2011) ISSN: 1557-3265 [Electronic] United States
PMID21355082 (Publication Type: Evaluation Study, Journal Article)
Chemical References
  • Antigens, Neoplasm
  • Biomarkers, Tumor
  • Cell Adhesion Molecules
  • DSG3 protein, human
  • Desmoglein 3
  • EPCAM protein, human
  • Epithelial Cell Adhesion Molecule
  • Parathyroid Hormone-Related Protein
Topics
  • Antigens, Neoplasm (genetics, metabolism)
  • Biomarkers, Tumor (genetics)
  • Carcinoma, Squamous Cell (diagnosis, secondary, surgery)
  • Cell Adhesion Molecules (genetics, metabolism)
  • Desmoglein 3 (genetics, metabolism)
  • Epithelial Cell Adhesion Molecule
  • Female
  • Head and Neck Neoplasms (pathology, surgery)
  • Humans
  • Intraoperative Care (methods)
  • Lymph Nodes (metabolism)
  • Lymphatic Metastasis
  • Male
  • Middle Aged
  • Parathyroid Hormone-Related Protein (genetics, metabolism)
  • ROC Curve
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sentinel Lymph Node Biopsy

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: