Saccharomyces cerevisiae decreases inflammatory responses induced by F4+ enterotoxigenic Escherichia coli in porcine intestinal epithelial cells.

Abstract

Probiotic yeasts may provide protection against intestinal inflammation induced by enteric pathogens. In piglets, infection with F4+ enterotoxigenic Escherichia coli (ETEC) leads to inflammation, diarrhea and intestinal damage. In this study, we investigated whether the yeast strains Saccharomyces cerevisiae (Sc, strain CNCM I-3856) and S. cerevisiae variety boulardii (Sb, strain CNCM I-3799) decreased the expression of pro-inflammatory cytokines and chemokines in intestinal epithelial IPI-2I cells cultured with F4+ ETEC. Results showed that viable Sc inhibited the ETEC-induced TNF-α gene expression whereas Sb did not. In contrast, killed Sc failed to inhibit the expression of pro-inflammatory genes. This inhibition was dependent on secreted soluble factors. Sc culture supernatant decreased the TNF-α, IL-1α, IL-6, IL-8, CXCL2 and CCL20 ETEC-induced mRNA. Furthermore, Sc culture supernatant filtrated fraction < 10 kDa displayed the same effects excepted for TNF-α. Thus, our results extended to Sc (strain CNCM I-3856) the inhibitory effects of some probiotic yeast strains onto inflammation.

• Authors: Galliano Zanello, François Meurens, Mustapha Berri, Claire Chevaleyre, Sandrine Melo, Eric Auclair, Henri Salmon
• Journal: Veterinary immunology and immunopathology (Vet Immunol Immunopathol) Vol. 141 Issue 1-2 Pg. 133-8 (May 15 2011) ISSN: 1873-2534 [Electronic] Netherlands
• PMID: 21354630 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Topics

• Animals
• Enterotoxigenic Escherichia coli (immunology)
• Escherichia coli Infections (immunology, microbiology, veterinary)
• Intestinal Mucosa (cytology, immunology, microbiology)
• Probiotics (therapeutic use)
• Reverse Transcriptase Polymerase Chain Reaction (veterinary)
• Saccharomyces cerevisiae (immunology)
• Swine (immunology, microbiology)
• Swine Diseases (immunology, microbiology, prevention & control)