Abstract |
In this study we have firstly compared a range of recombinant DNA poxvirus prime-boost immunisation strategies and shown that combined intramuscular (i.m.) 2× DNA-HIV/intranasal (i.n.) 2× FPV-HIV prime-boost immunisation can generate high-level of HIV-specific systemic (spleen) and mucosal (genito-rectal nodes, vaginal tissues and lung tissues) T cell responses and HIV-1 p24 Gag-specific serum IgG1, IgG2a and mucosal IgG, SIgA responses in vaginal secretions in BALB/c mice. Data indicate that following rDNA priming, two rFPV booster immunisations were necessary to generate good antibody and mucosal T cell immunity. This data also revealed that mucosal uptake of recombinant fowl pox (rFPV) was far superior to plasmid DNA. To further evaluate CD8+ T cell immunity, i.m. 2× DNA-HIV/i.n. 1× FPV-HIV immunisation strategy was directly compared with single shot poxvirus/poxvirus, i.n. FPV-HIV/i.m. VV-HIV immunisation. Results indicate that the latter strategy was able to generate strong sustained HIV-specific CD8+ T cells with higher avidity, broader cytokine/ chemokine profiles and better protection following influenza-K(d)Gag(197-205) challenge compared to rDNA poxvirus prime-boost strategy. Our findings further substantiate the importance of vector selection/combination, order and route of delivery when designing effective vaccines for HIV-1.
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Authors | Charani Ranasinghe, Fiona Eyers, John Stambas, David B Boyle, Ian A Ramshaw, Alistair J Ramsay |
Journal | Vaccine
(Vaccine)
Vol. 29
Issue 16
Pg. 3008-20
(Apr 05 2011)
ISSN: 1873-2518 [Electronic] Netherlands |
PMID | 21352941
(Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2011 Elsevier Ltd. All rights reserved. |
Chemical References |
- AIDS Vaccines
- HIV Antibodies
- HIV Core Protein p24
- Interleukin-2
- Vaccines, DNA
- p24 protein, Human Immunodeficiency Virus Type 1
- Interferon-gamma
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Topics |
- AIDS Vaccines
(immunology)
- Administration, Intranasal
- Animals
- CD8-Positive T-Lymphocytes
(immunology)
- Female
- Fowlpox virus
(immunology)
- HIV Antibodies
(blood)
- HIV Core Protein p24
(immunology)
- HIV Infections
(prevention & control)
- Immunity, Cellular
- Immunity, Humoral
- Immunity, Mucosal
- Immunization, Secondary
- Injections, Intramuscular
- Interferon-gamma
(immunology)
- Interleukin-2
(immunology)
- Mice
- Mice, Inbred BALB C
- Plasmids
(immunology)
- Vaccines, DNA
(immunology)
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