The effects of potassium channel opener P1075 on the human saphenous vein and human internal mammary artery.

Abstract

Because adrenergic contractions can contribute to the development of life-threatening spasm of coronary artery bypass graft, this study was performed to investigate the effect of adenosine 3-phosphate (ATP)-sensitive K channel (KATP) opener P1075 on contractions of isolated human saphenous vein (HSV) and human internal mammary artery (HIMA). Phasic contractions were evoked by electric field stimulation (20 Hz) and noradrenaline. The sustained contractions were evoked by phenylephrine. The presence of pore-forming Kir6.1 and Kir6.2 subunits of the KATP channels in the HIMA and only Kir6.2 in the HSV was confirmed immunomorphologically. P1075 inhibited in the HSV only, the electrical field stimulation contractions more strongly than noradrenaline contractions. In addition, the phenylephrine contractions of HSV were more sensitive to P1075 in comparison to those of HIMA. Glibenclamide, a KATP channel blocker antagonized the vasodilatation produced by P1075 in both grafts differently, because its effect was more prominent on the P1075-induced inhibition of contractions of HSV than of HIMA. We conclude that P1075 has a vasorelaxant effect and inhibited adrenergic contractions of the tested grafts. This effect is graft and vasoconstrictor selective and seems to be mediated by Kir6.1- and/or Kir6.2-containing KATP channels. Thus, P1075 can be considered as a potential drug in the prevention of graft spasm.

• Authors: Ljiljana C Gojkovic-Bukarica, Bojana B Beleslin-Cokic, Aleksandra N Novakovic, Miodrag S Peric, Jasmina Z Markovic-Lipkovski, Sanja Z Cirovic, Dusan G Nezic, Aleksandar R Lesic, Vladimir I Kanjuh, Helmut Heinle
• Journal: Journal of cardiovascular pharmacology (J Cardiovasc Pharmacol) Vol. 57 Issue 6 Pg. 648-55 (Jun 2011) ISSN: 1533-4023 [Electronic] United States
• PMID: 21346595 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Guanidines
• KATP Channels
• Kir6.2 channel
• Potassium Channel Blockers
• Potassium Channels, Inwardly Rectifying
• Protein Subunits
• Pyridines
• Vasodilator Agents
• uK-ATP-1 potassium channel
• N-cyano-N'-(1,1-dimethylpropyl)-N''-(3-pyridinyl)guanidine
• Glyburide

Topics

• Aged
• Electric Stimulation
• Glyburide (pharmacology)
• Guanidines (antagonists & inhibitors, pharmacology)
• Humans
• KATP Channels (agonists, metabolism)
• Male
• Mammary Arteries (cytology, drug effects, metabolism)
• Middle Aged
• Organ Culture Techniques
• Organ Specificity
• Osmolar Concentration
• Potassium Channel Blockers (pharmacology)
• Potassium Channels, Inwardly Rectifying (metabolism)
• Protein Subunits (metabolism)
• Pyridines (antagonists & inhibitors, pharmacology)
• Saphenous Vein (cytology, drug effects, metabolism)
• Vasoconstriction (drug effects)
• Vasodilation (drug effects)
• Vasodilator Agents (antagonists & inhibitors, pharmacology)