Characterization of mannose-binding lectin plasma levels and genetic polymorphisms in HIV-1-infected individuals.

Abstract

INTRODUCTION:

The present study investigated the association between mannose-binding lectin (MBL) gene polymorphism and serum levels with infection by HIV-1.

METHODS:

Blood samples (5 mL) were collected from 97 HIV-1-infected individuals resident in Belém, State of Pará, Brazil, who attended the Special Outpatient Unit for Infections and Parasitic Diseases (URE-DIPE). CD4+ T-lymphocyte count and plasma viral load were quantified. A 349bp fragment of exon 1 of the MBL was amplified via PCR, using genomic DNA extracted from controls and HIV-1-infected individuals, following established protocols. MBL plasma levels of the patients were quantified using an enzyme immunoassay kit.

RESULTS:

Two alleles were observed: MBL*O, with a frequency of 26.3% in HIV-1-infected individuals; and the wild allele MBL*A (73.7%). Similar frequencies were observed in the control group (p > 0.05). Genotype frequencies were distributed according to the Hardy-Weinberg equilibrium in both groups. Mean MBL plasma levels varied by genotype, with statistically significant differences between the AA and AO (p < 0.0001), and AA and OO (p < 0.001) genotypes, but not AO and OO (p = 0.17). Additionally, CD4+ T-lymphocytes and plasma viral load levels did not differ significantly by genotype (p > 0.05).

CONCLUSIONS:

The results of this study do not support the hypothesis that MBL gene polymorphism or low plasma MBL concentrations might have a direct influence on HIV-1 infection, although a broader study involving a large number of patients is needed.

• Authors: Antonio Carlos Rosário Vallinoto, Felipe Bonfim Freitas, Isabella Guirelli, Luiz Fernando Almeida Machado, Vânia Nakauth Azevedo, Izaura Cayres-Vallinoto, Marluísa Oliveira Guimarães Ishak, Ricardo Ishak
• Journal: Revista da Sociedade Brasileira de Medicina Tropical (Rev Soc Bras Med Trop) 2011 Jan-Feb Vol. 44 Issue 1 Pg. 1-3 ISSN: 1678-9849 [Electronic] Brazil
• PMID: 21340397 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Mannose-Binding Lectin

Topics

• Adult
• CD4 Lymphocyte Count
• Case-Control Studies
• HIV Infections (blood, genetics, virology)
• HIV-1 (genetics)
• Humans
• Mannose-Binding Lectin (blood, genetics)
• Polymerase Chain Reaction
• Polymorphism, Genetic (genetics)
• Viral Load