Abstract |
Kynurenic acid (KynA), a broad spectrum antagonist of excitatory amino acid receptors, may serve as a protective agent in neurological disorders. The potential anti-inflammatory effect of KynA in human leukocytes has not been characterized. The aim of this study was to compare the effects of KynA with those of a new analogue, 2-(2-N,N-dimethylaminoethylamine-1-carbonyl)-1H-quinolin-4-one hydrochloride on tumour necrosis factor-α (TNF-α) production and high mobility group box protein 1 ( HMGB1) secretion. The effects of KynA on granulocyte activation were investigated via the secretion of human neutrophil peptide 1-3 (HNP1-3). Peripheral blood mononuclear cells and granulocytes or CD14 positive monocytes were applied as effector cells, or whole blood cultures were used. TNF-α, HMGB1 and HNP1-3 concentrations were determined by ELISA, TNF-α and HNP1-3 mRNA expressions were quantified by reverse transcription PCR. KynA attenuated the TNF-α production of human mononuclear cells activated by heat-inactivated Staphylococcus aureus, inhibiting TNF-α production at the transcription level. Furthermore, KynA diminished HMGB1 secretion by U 937 monocytic cells and by peripheral blood monocytes. KynA inhibited the HNP1-3 secretion in whole blood and in granulocyte cultures. The suppressive effect of the KynA analogue was more potent than that of an equimolar concentration KynA in TNF-α, HMGB1 and HNP1-3 inhibition. These results suggest that the new KynA analogue has a more potent immunoregulatory effect than KynA on human mononuclear cells, monocytes and granulocytes and indicate the potential benefits of further exploration of its uses in human inflammatory disease.
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Authors | Zoltán Tiszlavicz, Balázs Németh, Ferenc Fülöp, László Vécsei, Katalin Tápai, Imre Ocsovszky, Yvette Mándi |
Journal | Naunyn-Schmiedeberg's archives of pharmacology
(Naunyn Schmiedebergs Arch Pharmacol)
Vol. 383
Issue 5
Pg. 447-55
(May 2011)
ISSN: 1432-1912 [Electronic] Germany |
PMID | 21336543
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 2-(2-N,N-dimethylaminoethylamine-1-carbonyl)-1H-quinolin-4-one
- HMGB1 Protein
- Quinolones
- Tumor Necrosis Factor-alpha
- alpha-Defensins
- human neutrophil peptide 1
- human neutrophil peptide 2
- human neutrophil peptide 3
- Kynurenic Acid
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Topics |
- Cells, Cultured
- Enzyme-Linked Immunosorbent Assay
- HMGB1 Protein
(antagonists & inhibitors, biosynthesis)
- Humans
- Kynurenic Acid
(analogs & derivatives, pharmacology)
- Leukocytes, Mononuclear
(drug effects, immunology)
- Monocytes
(drug effects, immunology)
- Neutrophils
(drug effects, immunology)
- Quinolones
(pharmacology)
- Reverse Transcriptase Polymerase Chain Reaction
- Tumor Necrosis Factor-alpha
(antagonists & inhibitors, biosynthesis)
- alpha-Defensins
(antagonists & inhibitors, metabolism)
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