HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Secretory leukocyte protease inhibitor plays an important role in the regulation of allergic asthma in mice.

Abstract
Secretory leukocyte protease inhibitor (SLPI) is an anti-inflammatory protein that is observed at high levels in asthma patients. Resiquimod, a TLR7/8 ligand, is protective against acute and chronic asthma, and it increases SLPI expression of macrophages in vitro. However, the protective role played by SLPI and the interactions between the SLPI and resiquimod pathways in the immune response occurring in allergic asthma have not been fully elucidated. To evaluate the role of SLPI in the development of asthma phenotypes and the effect of resiquimod treatment on SLPI, we assessed airway resistance and inflammatory parameters in the lungs of OVA-induced asthmatic SLPI transgenic and knockout mice and in mice treated with resiquimod. Compared with wild-type mice, allergic SLPI transgenic mice showed a decrease in lung resistance (p < 0.001), airway eosinophilia (p < 0.001), goblet cell hyperplasia (p < 0.001), and plasma IgE levels (p < 0.001). Allergic SLPI knockout mice displayed phenotype changes significantly more severe compared with wild-type mice. These phenotypes included lung resistance (p < 0.001), airway eosinophilia (p < 0.001), goblet cell hyperplasia (p < 0.001), cytokine levels in the lungs (p < 0.05), and plasma IgE levels (p < 0.001). Treatment of asthmatic transgenic mice with resiquimod increased the expression of SLPI and decreased inflammation in the lungs; resiquimod treatment was still effective in asthmatic SLPI knockout mice. Taken together, our study showed that the expression of SLPI protects against allergic asthma phenotypes, and treatment by resiquimod is independent of SLPI expression, displayed through the use of transgenic and knockout SLPI mice.
AuthorsRafael Marino, Thusanth Thuraisingam, Pierre Camateros, Cynthia Kanagaratham, Yong Zhong Xu, Jennifer Henri, Jingxuan Yang, Guoan He, Aihao Ding, Danuta Radzioch
JournalJournal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 186 Issue 7 Pg. 4433-42 (Apr 1 2011) ISSN: 1550-6606 [Electronic] United States
PMID21335488 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Allergens
  • Secretory Leukocyte Peptidase Inhibitor
Topics
  • Acute Disease
  • Allergens (administration & dosage)
  • Animals
  • Asthma (enzymology, immunology, pathology)
  • Bronchial Hyperreactivity (enzymology, immunology, physiopathology)
  • Cell Movement (genetics, immunology)
  • Disease Models, Animal
  • Inflammation (enzymology, genetics, prevention & control)
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Mice, Transgenic
  • Secretory Leukocyte Peptidase Inhibitor (biosynthesis, deficiency, physiology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: