Abstract | INTRODUCTION: METHODS: Adult ewes were chronically instrumented for a 24-h study and allocated to groups: sham: not injured, not treated, n = 6; control: injured, not treated, n = 6; and BBS-2: injured treated with iNOS dimerisation inhibitor BBS-2, n = 6. Control and BBS-2 groups received 40% total body surface area 3rd-degree cutaneous burn and cotton smoke insufflation into the lungs under isoflurane anaesthesia. RESULTS: Treatment with iNOS inhibitor BBS-2 significantly improved pulmonary gas exchange (partial pressure of oxygen in the blood/fraction of inspired oxygen (PaO₂/FiO₂) 409 ± 43 mmHg vs. 233 ± 50 mmHg in controls, p < 0.05) and reduced airway pressures (peak pressure 20 ± 1 cm H₂O vs. 28 ± 2 cm H₂O in controls, p < 0.05) and lung water content ( lung wet-to-dry ratio 4.1 ± 0.3 vs. 5.2 ± 0.2 in controls, p < 0.05) 24h after the burn and smoke injury. BBS-2 significantly reduced the increases in lung lymph nitrite/ nitrate (10 ± 3 μM vs. 26 ± 6 μM in controls, p < 0.05) and 3-nitrotyrosine (109 ± 11 (densitometry value) vs. 151 ± 18 in controls, p < 0.05). Burn/ smoke-induced increases in lung tissue nitrite/ nitrate, poly(ADP)ribose polymerase, nuclear factor-κB (NF-κB) activity, myeloperoxidase activity and malondialdehyde formation and interleukin (IL)-8 expression were also attenuated with BBS-2. CONCLUSIONS:
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Authors | Perenlei Enkhbaatar, Jianpu Wang, Fiona Saunders, Matthias Lange, Atsumori Hamahata, Sebastian Rehberg, John F Parkinson, Lillian D Traber, David N Herndon, Daniel L Traber |
Journal | Burns : journal of the International Society for Burn Injuries
(Burns)
Vol. 37
Issue 4
Pg. 638-45
(Jun 2011)
ISSN: 1879-1409 [Electronic] Netherlands |
PMID | 21334141
(Publication Type: Journal Article)
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Copyright | Copyright © 2011. Published by Elsevier Ltd. |
Chemical References |
- Imidazoles
- Interleukin-8
- N-((1,3-benzodioxol-5-yl)methyl)-1-(2-(1H-imidazol-1-yl)pyrimidin-4-yl)-4-(methoxycarbonyl)piperazine-2-acetamide
- NF-kappa B
- Piperazines
- Pyrimidines
- RNA, Messenger
- 3-nitrotyrosine
- Tyrosine
- Malondialdehyde
- Peroxidase
- Nitric Oxide Synthase Type II
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Topics |
- Analysis of Variance
- Animals
- Burns
(drug therapy, physiopathology)
- Disease Models, Animal
- Female
- Imidazoles
(therapeutic use)
- Immunohistochemistry
- Interleukin-8
(metabolism)
- Lung
(metabolism, pathology)
- Malondialdehyde
(metabolism)
- NF-kappa B
(metabolism)
- Nitric Oxide Synthase Type II
(antagonists & inhibitors)
- Peroxidase
(metabolism)
- Piperazines
(therapeutic use)
- Pulmonary Gas Exchange
(drug effects)
- Pyrimidines
(therapeutic use)
- RNA, Messenger
(metabolism)
- Sheep
- Smoke Inhalation Injury
(drug therapy, physiopathology)
- Tyrosine
(analogs & derivatives, metabolism)
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