Differential effects of inescapable stress on locus coeruleus GRK3, alpha2-adrenoceptor and CRF1 receptor levels in learned helpless and non-helpless rats: a potential link to stress resilience.

Abstract

Exposure of rats to unpredictable, inescapable stress results in two distinct behaviors during subsequent escape testing. One behavior, suggestive of lack of stress resilience, is prolonged escape latency compared to non-stressed rats and is labeled learned helplessness (LH). The other behavior suggestive of stress resilience is normal escape latency and is labeled non-helpless (NH). This study examines the effects of unpredictable, inescapable tail-shock stress (TSS) on alpha(2)-adrenoceptor (α(2A)-AR) and corticotropin-releasing factor 1 receptor (CRF(1)-R) regulation as well as protein levels of G protein-coupled receptor kinase 3 (GRK3), GRK2, tyrosine hydroxylase (TH) plus carbonylated protein levels in locus coeruleus (LC), amygdala (AMG), cortex (COR) and striatum (STR). In NH rats, α(2A)-AR and CRF(1)-R were significantly down-regulated in LC after TSS. No changes in these receptor levels were observed in the LC of LH rats. GRK3, which phosphorylates receptors and thereby contributes to α(2A)-AR and CRF(1)-R down-regulation, was reduced in the LC of LH but not NH rats. GRK2 levels were unchanged. In AMG, GRK3 but not GRK2 levels were reduced in LH but not NH rats, and receptor regulation was impaired in LH rats. In STR, no changes in GRK3 or GRK2 levels were observed. Finally, protein carbonylation, an index of oxidative stress, was increased in the LC and AMG of LH but not NH rats. We suggest that reduced stress resilience after TSS may be related to oxidative stress, depletion of GRK3 and impaired regulation of α(2A)-AR and CRF(1)-R in LC.

• Authors: Manish Taneja, Samina Salim, Kaustuv Saha, H Kevin Happe, Nidal Qutna, Frederick Petty, David B Bylund, Douglas C Eikenburg
• Journal: Behavioural brain research (Behav Brain Res) Vol. 221 Issue 1 Pg. 25-33 (Aug 01 2011) ISSN: 1872-7549 [Electronic] Netherlands
• PMID: 21333691 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier B.V. All rights reserved.

Chemical References

• Receptors, Adrenergic, alpha-2
• Receptors, Corticotropin-Releasing Hormone
• CRF receptor type 1
• Tyrosine 3-Monooxygenase
• G-Protein-Coupled Receptor Kinase 3
• Grk2 protein, rat
• Grk3 protein, rat
• G-Protein-Coupled Receptor Kinase 2

Topics

• Animals
• Brain (metabolism)
• Cluster Analysis
• Down-Regulation
• Electric Stimulation (methods)
• G-Protein-Coupled Receptor Kinase 2 (metabolism)
• G-Protein-Coupled Receptor Kinase 3 (metabolism)
• Helplessness, Learned
• Locus Coeruleus (metabolism)
• Male
• Protein Carbonylation
• Rats
• Rats, Sprague-Dawley
• Receptors, Adrenergic, alpha-2 (metabolism)
• Receptors, Corticotropin-Releasing Hormone (metabolism)
• Resilience, Psychological
• Stress, Psychological (metabolism)
• Tyrosine 3-Monooxygenase (metabolism)