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Indirect ELISA with recombinant GP5 for detecting antibodies to porcine reproductive and respiratory syndrome virus.

Abstract
Porcine reproductive and respiratory syndrome is caused by the PRRS virus (PRRSV), which has six structural proteins (GP2, GP3, GP4, GP5, M and N). GP5 and N protein are important targets for serological detection by enzyme-linked immunosorbent assay (ELISA) and other methods. Toward this goal, we developed an indirect ELISA with recombinant GP5 antigens and this method was validated by comparison to the LSI PRRSV-Ab ELISA kit. The results indicated that the optimal concentration of coated recombinant antigen was 0.2 μg/well for a serum dilution of 1:40. The rate of agreement with the LSI PRRSV-Ab kit was 88.7% (266/300). These results support the potential use of recombinant GP5 as an antigen for indirect ELISA to detect PRRSV antibodies in pigs.
AuthorsYan Chen, Hong Tian, Jian-hui He, Jin-yin Wu, You-jun Shang, Xiang-tao Liu
JournalVirologica Sinica (Virol Sin) Vol. 26 Issue 1 Pg. 61-6 (Feb 2011) ISSN: 1995-820X [Electronic] Netherlands
PMID21331892 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antibodies, Viral
  • Recombinant Proteins
  • Viral Envelope Proteins
  • glycoprotein 5, PRRSV
Topics
  • Animals
  • Antibodies, Viral (immunology)
  • Enzyme-Linked Immunosorbent Assay (methods)
  • Porcine respiratory and reproductive syndrome virus (genetics, immunology, metabolism)
  • Recombinant Proteins (genetics, immunology, metabolism)
  • Swine
  • Viral Envelope Proteins (genetics, immunology, metabolism)

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