Abstract |
Porcine reproductive and respiratory syndrome is caused by the PRRS virus (PRRSV), which has six structural proteins (GP2, GP3, GP4, GP5, M and N). GP5 and N protein are important targets for serological detection by enzyme-linked immunosorbent assay (ELISA) and other methods. Toward this goal, we developed an indirect ELISA with recombinant GP5 antigens and this method was validated by comparison to the LSI PRRSV-Ab ELISA kit. The results indicated that the optimal concentration of coated recombinant antigen was 0.2 μg/well for a serum dilution of 1:40. The rate of agreement with the LSI PRRSV-Ab kit was 88.7% (266/300). These results support the potential use of recombinant GP5 as an antigen for indirect ELISA to detect PRRSV antibodies in pigs.
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Authors | Yan Chen, Hong Tian, Jian-hui He, Jin-yin Wu, You-jun Shang, Xiang-tao Liu |
Journal | Virologica Sinica
(Virol Sin)
Vol. 26
Issue 1
Pg. 61-6
(Feb 2011)
ISSN: 1995-820X [Electronic] Netherlands |
PMID | 21331892
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies, Viral
- Recombinant Proteins
- Viral Envelope Proteins
- glycoprotein 5, PRRSV
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Topics |
- Animals
- Antibodies, Viral
(immunology)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Porcine respiratory and reproductive syndrome virus
(genetics, immunology, metabolism)
- Recombinant Proteins
(genetics, immunology, metabolism)
- Swine
- Viral Envelope Proteins
(genetics, immunology, metabolism)
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