Use of a hyperdried cross-linked amniotic membrane as initial therapy for corneal perforations.

To report the use of hyperdried cross-linked (HDCL) amniotic membrane (AM) patching with tissue adhesive as an initial therapy for corneal perforations.
Cryopreserved AM was cross-linked with 0.1% glutaraldehyde and then dried using far-infrared rays and microwaves (hyperdry method). Three eyes of three patients with corneal perforations of up to 3 mm in diameter were included in this study. They were treated with a single-layer patch of HDCL-AM applied with a tissue adhesive (2-octyl-cyanoacrylate). We also evaluated the resistance of HDCL-AM to collagenases during in vitro digestion testing.
In all three cases, the corneal perforations were repaired within 28 days (range, 17-28 days). No recurrence occurred during the follow-up period (3-6 months). In the collagenase digestion testing, the HDCL-AM did not dissolve until 48 h, whereas the cryopreserved AM completely dissolved within 60 min.
Three cases of corneal perforations were successfully managed using HDCL-AM patching with tissue adhesive. The HDCL-AM was resistant to collagenases during in vitro digestion testing. The HDCL-AM was a useful substrate for corneal perforations. This simple surgical technique may be one of the initial therapeutic options for corneal perforations.
AuthorsKiyotaka Kitagawa, Motonori Okabe, Shuichiro Yanagisawa, Xue-Yun Zhang, Toshio Nikaido, Atsushi Hayashi
JournalJapanese journal of ophthalmology (Jpn J Ophthalmol) Vol. 55 Issue 1 Pg. 16-21 (Jan 2011) ISSN: 1613-2246 [Electronic] Japan
PMID21331687 (Publication Type: Case Reports, Journal Article)
Copyright© Japanese Ophthalmological Society 2011.
Chemical References
  • Cross-Linking Reagents
  • Cyanoacrylates
  • Tissue Adhesives
  • octyl 2-cyanoacrylate
  • Collagenases
  • Glutaral
  • Adolescent
  • Adult
  • Aged
  • Amnion (chemistry, drug effects)
  • Biological Dressings
  • Collagenases (pharmacology)
  • Corneal Perforation (therapy)
  • Cross-Linking Reagents (pharmacology)
  • Cyanoacrylates (therapeutic use)
  • Desiccation
  • Female
  • Glutaral (pharmacology)
  • Humans
  • Male
  • Time Factors
  • Tissue Adhesives (therapeutic use)
  • Wound Healing

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