Use of a hyperdried cross-linked amniotic membrane as initial therapy for corneal perforations.

Abstract

PURPOSE:

To report the use of hyperdried cross-linked (HDCL) amniotic membrane (AM) patching with tissue adhesive as an initial therapy for corneal perforations.

METHODS:

Cryopreserved AM was cross-linked with 0.1% glutaraldehyde and then dried using far-infrared rays and microwaves (hyperdry method). Three eyes of three patients with corneal perforations of up to 3 mm in diameter were included in this study. They were treated with a single-layer patch of HDCL-AM applied with a tissue adhesive (2-octyl-cyanoacrylate). We also evaluated the resistance of HDCL-AM to collagenases during in vitro digestion testing.

RESULTS:

In all three cases, the corneal perforations were repaired within 28 days (range, 17-28 days). No recurrence occurred during the follow-up period (3-6 months). In the collagenase digestion testing, the HDCL-AM did not dissolve until 48 h, whereas the cryopreserved AM completely dissolved within 60 min.

CONCLUSIONS:

Three cases of corneal perforations were successfully managed using HDCL-AM patching with tissue adhesive. The HDCL-AM was resistant to collagenases during in vitro digestion testing. The HDCL-AM was a useful substrate for corneal perforations. This simple surgical technique may be one of the initial therapeutic options for corneal perforations.

• Authors: Kiyotaka Kitagawa, Motonori Okabe, Shuichiro Yanagisawa, Xue-Yun Zhang, Toshio Nikaido, Atsushi Hayashi
• Journal: Japanese journal of ophthalmology (Jpn J Ophthalmol) Vol. 55 Issue 1 Pg. 16-21 (Jan 2011) ISSN: 1613-2246 [Electronic] Japan
• PMID: 21331687 (Publication Type: Case Reports, Journal Article)
• Copyright: © Japanese Ophthalmological Society 2011.

Chemical References

• Cross-Linking Reagents
• Cyanoacrylates
• Tissue Adhesives
• octyl 2-cyanoacrylate
• Collagenases
• Glutaral

Topics

• Adolescent
• Adult
• Aged
• Amnion (chemistry, drug effects)
• Biological Dressings
• Collagenases (pharmacology)
• Corneal Perforation (therapy)
• Cross-Linking Reagents (pharmacology)
• Cyanoacrylates (therapeutic use)
• Desiccation
• Female
• Glutaral (pharmacology)
• Humans
• Male
• Time Factors
• Tissue Adhesives (therapeutic use)
• Wound Healing