Abstract | BACKGROUND: METHOD: We studied 16 patients with preeclampsia (systolic BP=150±4 mmHg; 28±2 years, 37±1 weeks gestational age) and 14 gestational age-matched normal pregnant women (systolic BP=112±2 mmHg). RESULTS:
Preeclampsia was associated with a rise in plasma and placental levels of MBG. In preeclamptic umbilical arteries, the expression of Fli-1, a transcription factor and a negative regulator of fibrosis, was significantly reduced (P<0.001), whereas procollagen-1 expression was increased (P<0.01). As compared to control vessels, isolated rings of umbilical arteries from patients with preeclampsia demonstrated unaltered responsiveness to endothelin-1 (EC50=2.2 and 3.2 nmol/l, respectively), but exhibited an impaired response to the relaxant effect of sodium nitroprusside (EC50=1.5 vs. 32.4 nmol/l, P<.001) following endothelin-1-induced constriction. Ex-vivo treatment of normal umbilical arteries explants with 1 and 10 nmol/l MBG for 24 h mimicked the effects of preeclampsia, specifically suppressed Fli-1 and increased collagen-1 expression while impairing vasorelaxation. CONCLUSION: Our results indicate that in preeclampsia, elevated levels of MBG induce vascular fibrosis via a Fli-1-dependent mechanism which leads to an impairment of vasorelaxation, and suggest that MBG represents a potential target for therapy of this syndrome.
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Authors | Elena R Nikitina, Anton V Mikhailov, Ekaterina S Nikandrova, Elena V Frolova, Artem V Fadeev, Vera V Shman, Victoria Y Shilova, Natalia I Tapilskaya, Joseph I Shapiro, Olga V Fedorova, Alexei Y Bagrov |
Journal | Journal of hypertension
(J Hypertens)
Vol. 29
Issue 4
Pg. 769-76
(Apr 2011)
ISSN: 1473-5598 [Electronic] England |
PMID | 21330936
(Publication Type: Journal Article, Research Support, N.I.H., Intramural)
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Chemical References |
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Topics |
- Adult
- Blotting, Western
- Female
- Humans
- Immunoassay
- Pre-Eclampsia
(metabolism)
- Pregnancy
- Steroids
(metabolism)
- Umbilical Arteries
(metabolism, pathology, physiopathology)
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