Inhibition of
histone deacetylases (HDAC) has been shown to modulate gene expression and
cytokine production after stimulation with several stimuli. In the present study, the antiinflammatory effect of a potent HDACi,
ITF2357, was explored in different experimental models of
arthritis. In addition, the bone protective effect of
ITF2357 was investigated in vitro. Treatment of acute
arthritis (Streptococcus pyogenes cell wall [SCW]
arthritis) with
ITF2357 showed that joint swelling and cell influx into the joint cavity were reduced. Furthermore, the chondrocyte metabolic function was improved by treatment of
ITF2357. The production of proinflammatory
cytokines by synovial tissue was reduced after
ITF2357 treatment. To examine the effect of HDAC inhibition on joint destruction,
ITF2357 was applied to both rat
adjuvant arthritis and mouse
collagen type II arthritis.
ITF2357 treatment both ameliorates the severity scores in
arthritis models and prevents bone destruction. In an in vitro bone destruction assay,
ITF2357 was highly effective at a dose of 100 nmol/L. In conclusion, inhibition of HDAC prevents joint
inflammation and cartilage and bone destruction in
experimental arthritis.