Passive immune-protection of small abalone against Vibrio alginolyticus infection by anti-Vibrio IgY-encapsulated feed.

Abstract

Small abalone (Haliotis diversicolor supertexta) is a high value-added shellfish. It however has been suffering Vibrio alginolyticus infections, which cause mass death of small abalone and thus great economic losses, particularly in artificial aquaculture. In this study, we attempted to treat small abalone with anti-Vibrio IgY to elicit a passive immunity directly against V. alginolyticus infections. Anti-Vibrio IgY was alginate encapsulated in egg powders as feed, which may avoid antibody inactivation in the gastrointestinal tract of small abalone. The feed was tested for the stability of anti-Vibrio IgY in a gastrointestinal mimic environment. The result showed anti-Vibrio IgY retained activity as high as 90% after 4 h exposure to pancreatic enzymes. Addition of 0, 5 or 10% anti-Vibrio IgY-encapsulated egg powders into a basal diet to form abalone diet formulae. Small abalones fed with the anti-Vibrio IgY formulae showed a relatively high respiratory burst activity than those without anti-Vibrio IgY treatments. The survival rates of small abalones fed with 5 or 10% anti-Vibrio IgY egg powders were in the range of 65-70% 14 days post-V. alginolyticus challenge (1 x 10⁶ c.f.u.), which was significantly higher than 0% of those fed without anti-Vibrio IgY. The anti-Vibrio IgY-encapsulated formulae were thus concluded to be an effective means to prevent small abalone from V. alginolyticus infection, and may be practical in use in abalone aquaculture.

• Authors: Chang-Jer Wu, Hang Wang, Yi-Lin Chan, Tsung-Lin Li
• Journal: Fish & shellfish immunology (Fish Shellfish Immunol) 2011 Apr-May Vol. 30 Issue 4-5 Pg. 1042-8 ISSN: 1095-9947 [Electronic] England
• PMID: 21300158 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2011 Elsevier Ltd. All rights reserved.

Chemical References

• Alginates
• Antibodies, Bacterial
• IgY
• Immunoglobulins

Topics

• Alginates (pharmacology)
• Animals
• Antibodies, Bacterial (immunology)
• Aquaculture
• Chickens
• Female
• Immunization (methods)
• Immunoglobulins (immunology)
• Kaplan-Meier Estimate
• Mollusca (immunology, virology)
• Vibrio Infections (immunology, prevention & control)
• Vibrio alginolyticus (immunology)